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shotgun-sequencing

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Metagenomic functional profiling pipeline for reconstructing metabolic pathway abundances and gene families from shotgun sequencing reads. Automates marker-based taxonomic profiling and translated search to quantify community-wide metabolic potential and taxonomically attribute metabolic functions.

  • Updated Jul 5, 2026
  • Shell

A reproducible QIIME 2 MOSHPIT pipeline that assembles, bins, dereplicates and taxonomically classifies whole‑metagenome data. Includes MEGAHIT assembly, MetaBAT 2 binning, BUSCO quality control, Sourmash dereplication and Kraken 2/Bracken abundance estimation. Ideal for microbial‑ecology, functional‑genomics and strain‑level profiling studies.

  • Updated Jun 5, 2026
  • Python

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