Trajectories with Monocle 3 and Seurat 4 #4398
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jintaoxu07
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Hi,
When using the as.cell_data_set function in SeuratWrappers, will any gene expression data (gene names, expression level) from the Seurat object also be transferred so that a differential expressed gene can be analyzed in monocle3?
I am asking because I followed the "Calculating Trajectories with Monocle 3 and Seurat" and I do get the pseudotime analysis done for the seurat object. Next, I would like to find genes that change as a function of pseudotime. I am trying to follow the monocle3 demonstration, however, when I plot several genes that should be expressed by my cells using the plot_cells function in monocle 3, it says these genes are not founded.
Do you have any suggestions on how to get this part done?
Thank you very much for your help!
Best,
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