- Documentation available at: https://msci.readthedocs.io.
- Web application available at: msci--proteomicsunit.streamlit.app
Peptide identification by mass spectrometry relies on the interpretation of fragmentation spectra based on the m/z pattern, relative intensities, and retention time (RT). Given a proteome, we wondered how many peptides generate very similar fragmentation spectra with current MS methods. MSCI is a Python package built to assess the information content of peptide fragmentation spectra, we aimed calculating an information-content index for all peptides in a given proteome would enable us to design data acquisition and data analysis strategies that generate and prioritize the most informative fragment ions to be queried for peptide quantification.
You can install MSCI directly using pip, which will also handle the necessary dependencies.
pip install MSCI==0.2.3
Open the Notebook: Click on the following Tutorial_google_colab
If you would like to contribute to this project, feel free to fork the repository on GitHub and submit a pull request.