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Version 0.0.6 - new function, new parameters, bugs fixed

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@hansenjn hansenjn released this 25 Feb 08:30
· 141 commits to master since this release

Newly implemented

  • New function that allows an increased range for connecting neighbored voxels to a cilium or to an intensity region: if selected in the initial settings dialog (see red marks in second image below), also diagonally adjacent voxels (see scheme below: orange pixels in are diagonally adjacent to the white pixel in the center) are linked in the Flood Filling algorithm.

20200225_PixelRange_CiliaQ_red

v006_new_settings

  • New parameter integrated intensity for channels A and B: determined as the sum of all intensities detected as the intensity profile and represents the total amount of the "label" in the cilium.
  • New paramter average intensity on center line for channels A and B: determined as the average of all intensities detected as the intensity profile and might represent a more stable detection method for determinig the average localization of a "label" to the cilium compared to the parameter average intensity.
  • New output: channel thresholds also output in results outputs for each cilium.
  • 3D visualizations are now saved as PNG (and not as TIF any more), reducing storage space of CiliaQ output data.

Bugs fixed

  • In v0.0.5 the filtering of particles in intensity regions did not work for all frames after the first frame in the timelapse mode.
  • Output profiles were incorrect in v0.0.5 - sometimes the profile points were not in consecutive order on the cilium, resulting in 0s in the output intensity profiles and resulting in wrong determination of the bending index and orientation vectors.
  • Basal stain detection at the ciliary base and tip was wrong in v0.0.5.