DNA Mutation Detection Tool

A Python-based tool for detecting point mutations, insertions, and deletions in DNA sequences by comparing reference and mutated sequences from FASTA files.

Features

• Reads DNA sequences from FASTA files
• Detects different types of mutations:
  • Point mutations (single nucleotide changes)
  • Insertions
  • Deletions
• Generates detailed mutation reports
• Saves analysis results to a text file

Requirements

• Python 3.x
• FASTA format DNA sequence files

Installation

1. Clone the repository:

git clone https://github.com/your-username/dna-mutation-detector.git
cd dna-mutation-detector

2. Make sure your FASTA files are in the data directory:
   • reference_sequence.fasta: Contains the reference DNA sequence
   • mutated_sequences.fasta: Contains the mutated DNA sequence

Usage

Run the program:

python main.py

The program will:

1. Read both DNA sequences from the FASTA files
2. Compare the sequences and detect mutations
3. Display the results on screen
4. Save a detailed report to a text file

Output

The program generates a report file named dna_analysis_YYYYMMDD_HHMMSS.txt containing:

• Original and mutated DNA sequences
• List of detected mutations
• Position of each mutation
• Type of mutation
• Detailed description of changes

Example

Input FASTA files:

>reference
ATCGATCGATCG
>mutated
ATCGATCGATCA

Output:

Found Mutations:
------------------------------------------------------------
Position: 12
Original: G
Mutated:  A
Type:     Point Mutation
Description: G -> A change
------------------------------------------------------------

Contributing

Feel free to submit issues and enhancement requests!

License

This project is licensed under the MIT License - see the LICENSE file for details.