Genomic analysis pipeline

                            

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Identify mutations related to resistance

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1. Check the quality of FASTQ files
2. Remove low-quality reads
3. Remove duplicates
4. Map against the H37Rv genome or perform De Novo assembly
5. Variant calling (SNPs and indels)
6. Classify resistance-related mutations
7. Compare with phenotypic data for validation

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Investigate New Mutations

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1. Check the quality of FASTQ files
2. Remove low-quality reads
3. Remove duplicates
4. Map against the H37Rv genome or perform De Novo assembly
5. Variant calling (SNPs and indels)
6. Identify variants not listed in databases
7. Associate with specific genes

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Analyze Genomic Variability

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1. Check the quality of FASTQ files
2. Remove low-quality reads
3. Remove duplicates
4. Map against the H37Rv genome or perform De Novo assembly
5. Phylogenetic analysis
6. Build phylogenetic trees
7. Genome comparison
8. Analysis of conserved and convergent regions

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Explore Potential Therapeutic Targets

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1. Check the quality of FASTQ files
2. Remove low-quality reads
3. Remove duplicates
4. Map against the H37Rv genome or perform De Novo assembly
5. Identification of specific genes
6. Structural protein modeling
7. Virtual screening
8. Simulate interaction with drugs

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Key Features:

• Interactive Workflow: Click on the images to navigate to the corresponding section.
• Back to Top Links: Each section includes a link to return to the top of the page.
• Clear Steps: Each step is outlined in a simple, easy-to-follow format.

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For any questions, please contact me by email: [email protected]

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