Here is a brief list of updates:
- The pipeline now uses
.csvfile instead of.tsvas an input. I noticed that it was challenging for users outside of cellgeni to generate those - Removed
--mapperand--solo_quantparameters as pipeline now handles any type of input:CellRanger/STARsolooutput directory (works for all flavors ofCellRanger),.h5file,.mtxdirectory. For more details seeexamples/sample_table.csvfile. - Added
--mapper_presetoption to choose parameters for CellBender fromCellRanger/STARsolofiltering (needs full output directory) - You can provide several modalities to
--exclude_features "Peaks,Multiplexing Capture,CRISPR Guide Capture"parameter now - Added
--ignore_extensionsparameter to ignore some any file types when loading data from iRODS. Targeted at heavy.bamfiles - I also converted cellbender and irods processes to nf-core and added some tests for them. You can find all test data on iRODS:
/archive/cellgeni/tests
For more details read README.md file