diff --git a/tests/bsim/bluetooth/mesh/src/mesh_test.c b/tests/bsim/bluetooth/mesh/src/mesh_test.c
index 658aa48133b54..7226c8222366e 100644
--- a/tests/bsim/bluetooth/mesh/src/mesh_test.c
+++ b/tests/bsim/bluetooth/mesh/src/mesh_test.c
@@ -292,9 +292,6 @@ void bt_mesh_device_setup(const struct bt_mesh_prov *prov, const struct bt_mesh_
 
 	if (IS_ENABLED(CONFIG_BT_SETTINGS)) {
 		LOG_INF("Loading stored settings");
-		if (IS_ENABLED(CONFIG_PSA_CRYPTO_PROVIDER_MBEDTLS)) {
-			settings_load_subtree("itsemul");
-		}
 		settings_load_subtree("bt");
 	}