diff --git a/xarray/backends/zarr.py b/xarray/backends/zarr.py
index 9796fcbf9e2..c222f7f5b14 100644
--- a/xarray/backends/zarr.py
+++ b/xarray/backends/zarr.py
@@ -1223,9 +1223,13 @@ def open_datatree(
         from xarray.core.treenode import NodePath
 
         filename_or_obj = _normalize_path(filename_or_obj)
+        if storage_options:
+            kwargs["backend_kwargs"] = {"storage_options": storage_options}
         if group:
             parent = NodePath("/") / NodePath(group)
-            stores = ZarrStore.open_store(filename_or_obj, group=parent)
+            stores = ZarrStore.open_store(
+                filename_or_obj, group=parent, storage_options=storage_options
+            )
             if not stores:
                 ds = open_dataset(
                     filename_or_obj, group=parent, engine="zarr", **kwargs
@@ -1233,7 +1237,10 @@ def open_datatree(
                 return DataTree.from_dict({str(parent): ds})
         else:
             parent = NodePath("/")
-            stores = ZarrStore.open_store(filename_or_obj, group=parent)
+            stores = ZarrStore.open_store(
+                filename_or_obj, group=parent, storage_options=storage_options
+            )
+
         ds = open_dataset(filename_or_obj, group=parent, engine="zarr", **kwargs)
         tree_root = DataTree.from_dict({str(parent): ds})
         for path_group, store in stores.items():