diff --git a/.github/workflows/core_contrib_test_0.yml b/.github/workflows/core_contrib_test_0.yml
index cbe61bbf5e..c003de5050 100644
--- a/.github/workflows/core_contrib_test_0.yml
+++ b/.github/workflows/core_contrib_test_0.yml
@@ -877,8 +877,8 @@ jobs:
       - name: Run tests
         run: tox -e py38-test-instrumentation-requests -- -ra
 
-  py38-test-instrumentation-starlette:
-    name: instrumentation-starlette 
+  py38-test-instrumentation-starlette-oldest:
+    name: instrumentation-starlette-oldest 
     runs-on: ubuntu-latest
     steps:
       - name: Checkout contrib repo @ SHA - ${{ env.CONTRIB_REPO_SHA }}
@@ -897,7 +897,29 @@ jobs:
         run: pip install tox-uv
 
       - name: Run tests
-        run: tox -e py38-test-instrumentation-starlette -- -ra
+        run: tox -e py38-test-instrumentation-starlette-oldest -- -ra
+
+  py38-test-instrumentation-starlette-latest:
+    name: instrumentation-starlette-latest 
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout contrib repo @ SHA - ${{ env.CONTRIB_REPO_SHA }}
+        uses: actions/checkout@v4
+        with:
+          repository: open-telemetry/opentelemetry-python-contrib
+          ref: ${{ env.CONTRIB_REPO_SHA }}
+
+      - name: Set up Python 3.8
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.8"
+          architecture: "x64"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e py38-test-instrumentation-starlette-latest -- -ra
 
   py38-test-instrumentation-jinja2:
     name: instrumentation-jinja2 
diff --git a/.github/workflows/test_1.yml b/.github/workflows/test_1.yml
index e3128205c1..39850dd74f 100644
--- a/.github/workflows/test_1.yml
+++ b/.github/workflows/test_1.yml
@@ -286,8 +286,8 @@ jobs:
       - name: Run tests
         run: tox -e py313-test-instrumentation-requests -- -ra
 
-  py38-test-instrumentation-starlette_ubuntu-latest:
-    name: instrumentation-starlette 3.8 Ubuntu
+  py38-test-instrumentation-starlette-oldest_ubuntu-latest:
+    name: instrumentation-starlette-oldest 3.8 Ubuntu
     runs-on: ubuntu-latest
     steps:
       - name: Checkout repo @ SHA - ${{ github.sha }}
@@ -302,10 +302,28 @@ jobs:
         run: pip install tox-uv
 
       - name: Run tests
-        run: tox -e py38-test-instrumentation-starlette -- -ra
+        run: tox -e py38-test-instrumentation-starlette-oldest -- -ra
 
-  py39-test-instrumentation-starlette_ubuntu-latest:
-    name: instrumentation-starlette 3.9 Ubuntu
+  py38-test-instrumentation-starlette-latest_ubuntu-latest:
+    name: instrumentation-starlette-latest 3.8 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python 3.8
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.8"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e py38-test-instrumentation-starlette-latest -- -ra
+
+  py39-test-instrumentation-starlette-oldest_ubuntu-latest:
+    name: instrumentation-starlette-oldest 3.9 Ubuntu
     runs-on: ubuntu-latest
     steps:
       - name: Checkout repo @ SHA - ${{ github.sha }}
@@ -320,10 +338,46 @@ jobs:
         run: pip install tox-uv
 
       - name: Run tests
-        run: tox -e py39-test-instrumentation-starlette -- -ra
+        run: tox -e py39-test-instrumentation-starlette-oldest -- -ra
 
-  py310-test-instrumentation-starlette_ubuntu-latest:
-    name: instrumentation-starlette 3.10 Ubuntu
+  py39-test-instrumentation-starlette-latest_ubuntu-latest:
+    name: instrumentation-starlette-latest 3.9 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python 3.9
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.9"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e py39-test-instrumentation-starlette-latest -- -ra
+
+  py310-test-instrumentation-starlette-oldest_ubuntu-latest:
+    name: instrumentation-starlette-oldest 3.10 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python 3.10
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.10"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e py310-test-instrumentation-starlette-oldest -- -ra
+
+  py310-test-instrumentation-starlette-latest_ubuntu-latest:
+    name: instrumentation-starlette-latest 3.10 Ubuntu
     runs-on: ubuntu-latest
     steps:
       - name: Checkout repo @ SHA - ${{ github.sha }}
@@ -338,10 +392,10 @@ jobs:
         run: pip install tox-uv
 
       - name: Run tests
-        run: tox -e py310-test-instrumentation-starlette -- -ra
+        run: tox -e py310-test-instrumentation-starlette-latest -- -ra
 
-  py311-test-instrumentation-starlette_ubuntu-latest:
-    name: instrumentation-starlette 3.11 Ubuntu
+  py311-test-instrumentation-starlette-oldest_ubuntu-latest:
+    name: instrumentation-starlette-oldest 3.11 Ubuntu
     runs-on: ubuntu-latest
     steps:
       - name: Checkout repo @ SHA - ${{ github.sha }}
@@ -356,10 +410,46 @@ jobs:
         run: pip install tox-uv
 
       - name: Run tests
-        run: tox -e py311-test-instrumentation-starlette -- -ra
+        run: tox -e py311-test-instrumentation-starlette-oldest -- -ra
 
-  py312-test-instrumentation-starlette_ubuntu-latest:
-    name: instrumentation-starlette 3.12 Ubuntu
+  py311-test-instrumentation-starlette-latest_ubuntu-latest:
+    name: instrumentation-starlette-latest 3.11 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python 3.11
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.11"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e py311-test-instrumentation-starlette-latest -- -ra
+
+  py312-test-instrumentation-starlette-oldest_ubuntu-latest:
+    name: instrumentation-starlette-oldest 3.12 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python 3.12
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.12"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e py312-test-instrumentation-starlette-oldest -- -ra
+
+  py312-test-instrumentation-starlette-latest_ubuntu-latest:
+    name: instrumentation-starlette-latest 3.12 Ubuntu
     runs-on: ubuntu-latest
     steps:
       - name: Checkout repo @ SHA - ${{ github.sha }}
@@ -374,10 +464,28 @@ jobs:
         run: pip install tox-uv
 
       - name: Run tests
-        run: tox -e py312-test-instrumentation-starlette -- -ra
+        run: tox -e py312-test-instrumentation-starlette-latest -- -ra
+
+  py313-test-instrumentation-starlette-oldest_ubuntu-latest:
+    name: instrumentation-starlette-oldest 3.13 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python 3.13
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.13"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e py313-test-instrumentation-starlette-oldest -- -ra
 
-  py313-test-instrumentation-starlette_ubuntu-latest:
-    name: instrumentation-starlette 3.13 Ubuntu
+  py313-test-instrumentation-starlette-latest_ubuntu-latest:
+    name: instrumentation-starlette-latest 3.13 Ubuntu
     runs-on: ubuntu-latest
     steps:
       - name: Checkout repo @ SHA - ${{ github.sha }}
@@ -392,10 +500,28 @@ jobs:
         run: pip install tox-uv
 
       - name: Run tests
-        run: tox -e py313-test-instrumentation-starlette -- -ra
+        run: tox -e py313-test-instrumentation-starlette-latest -- -ra
+
+  pypy3-test-instrumentation-starlette-oldest_ubuntu-latest:
+    name: instrumentation-starlette-oldest pypy-3.8 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python pypy-3.8
+        uses: actions/setup-python@v5
+        with:
+          python-version: "pypy-3.8"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e pypy3-test-instrumentation-starlette-oldest -- -ra
 
-  pypy3-test-instrumentation-starlette_ubuntu-latest:
-    name: instrumentation-starlette pypy-3.8 Ubuntu
+  pypy3-test-instrumentation-starlette-latest_ubuntu-latest:
+    name: instrumentation-starlette-latest pypy-3.8 Ubuntu
     runs-on: ubuntu-latest
     steps:
       - name: Checkout repo @ SHA - ${{ github.sha }}
@@ -410,7 +536,7 @@ jobs:
         run: pip install tox-uv
 
       - name: Run tests
-        run: tox -e pypy3-test-instrumentation-starlette -- -ra
+        run: tox -e pypy3-test-instrumentation-starlette-latest -- -ra
 
   py38-test-instrumentation-jinja2_ubuntu-latest:
     name: instrumentation-jinja2 3.8 Ubuntu
@@ -4389,129 +4515,3 @@ jobs:
 
       - name: Run tests
         run: tox -e py312-test-instrumentation-tornado -- -ra
-
-  py313-test-instrumentation-tornado_ubuntu-latest:
-    name: instrumentation-tornado 3.13 Ubuntu
-    runs-on: ubuntu-latest
-    steps:
-      - name: Checkout repo @ SHA - ${{ github.sha }}
-        uses: actions/checkout@v4
-
-      - name: Set up Python 3.13
-        uses: actions/setup-python@v5
-        with:
-          python-version: "3.13"
-
-      - name: Install tox
-        run: pip install tox-uv
-
-      - name: Run tests
-        run: tox -e py313-test-instrumentation-tornado -- -ra
-
-  pypy3-test-instrumentation-tornado_ubuntu-latest:
-    name: instrumentation-tornado pypy-3.8 Ubuntu
-    runs-on: ubuntu-latest
-    steps:
-      - name: Checkout repo @ SHA - ${{ github.sha }}
-        uses: actions/checkout@v4
-
-      - name: Set up Python pypy-3.8
-        uses: actions/setup-python@v5
-        with:
-          python-version: "pypy-3.8"
-
-      - name: Install tox
-        run: pip install tox-uv
-
-      - name: Run tests
-        run: tox -e pypy3-test-instrumentation-tornado -- -ra
-
-  py38-test-instrumentation-tortoiseorm_ubuntu-latest:
-    name: instrumentation-tortoiseorm 3.8 Ubuntu
-    runs-on: ubuntu-latest
-    steps:
-      - name: Checkout repo @ SHA - ${{ github.sha }}
-        uses: actions/checkout@v4
-
-      - name: Set up Python 3.8
-        uses: actions/setup-python@v5
-        with:
-          python-version: "3.8"
-
-      - name: Install tox
-        run: pip install tox-uv
-
-      - name: Run tests
-        run: tox -e py38-test-instrumentation-tortoiseorm -- -ra
-
-  py39-test-instrumentation-tortoiseorm_ubuntu-latest:
-    name: instrumentation-tortoiseorm 3.9 Ubuntu
-    runs-on: ubuntu-latest
-    steps:
-      - name: Checkout repo @ SHA - ${{ github.sha }}
-        uses: actions/checkout@v4
-
-      - name: Set up Python 3.9
-        uses: actions/setup-python@v5
-        with:
-          python-version: "3.9"
-
-      - name: Install tox
-        run: pip install tox-uv
-
-      - name: Run tests
-        run: tox -e py39-test-instrumentation-tortoiseorm -- -ra
-
-  py310-test-instrumentation-tortoiseorm_ubuntu-latest:
-    name: instrumentation-tortoiseorm 3.10 Ubuntu
-    runs-on: ubuntu-latest
-    steps:
-      - name: Checkout repo @ SHA - ${{ github.sha }}
-        uses: actions/checkout@v4
-
-      - name: Set up Python 3.10
-        uses: actions/setup-python@v5
-        with:
-          python-version: "3.10"
-
-      - name: Install tox
-        run: pip install tox-uv
-
-      - name: Run tests
-        run: tox -e py310-test-instrumentation-tortoiseorm -- -ra
-
-  py311-test-instrumentation-tortoiseorm_ubuntu-latest:
-    name: instrumentation-tortoiseorm 3.11 Ubuntu
-    runs-on: ubuntu-latest
-    steps:
-      - name: Checkout repo @ SHA - ${{ github.sha }}
-        uses: actions/checkout@v4
-
-      - name: Set up Python 3.11
-        uses: actions/setup-python@v5
-        with:
-          python-version: "3.11"
-
-      - name: Install tox
-        run: pip install tox-uv
-
-      - name: Run tests
-        run: tox -e py311-test-instrumentation-tortoiseorm -- -ra
-
-  py312-test-instrumentation-tortoiseorm_ubuntu-latest:
-    name: instrumentation-tortoiseorm 3.12 Ubuntu
-    runs-on: ubuntu-latest
-    steps:
-      - name: Checkout repo @ SHA - ${{ github.sha }}
-        uses: actions/checkout@v4
-
-      - name: Set up Python 3.12
-        uses: actions/setup-python@v5
-        with:
-          python-version: "3.12"
-
-      - name: Install tox
-        run: pip install tox-uv
-
-      - name: Run tests
-        run: tox -e py312-test-instrumentation-tortoiseorm -- -ra
diff --git a/.github/workflows/test_2.yml b/.github/workflows/test_2.yml
index 3b796e31d5..4d6c81fe51 100644
--- a/.github/workflows/test_2.yml
+++ b/.github/workflows/test_2.yml
@@ -16,6 +16,132 @@ env:
 
 jobs:
 
+  py313-test-instrumentation-tornado_ubuntu-latest:
+    name: instrumentation-tornado 3.13 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python 3.13
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.13"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e py313-test-instrumentation-tornado -- -ra
+
+  pypy3-test-instrumentation-tornado_ubuntu-latest:
+    name: instrumentation-tornado pypy-3.8 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python pypy-3.8
+        uses: actions/setup-python@v5
+        with:
+          python-version: "pypy-3.8"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e pypy3-test-instrumentation-tornado -- -ra
+
+  py38-test-instrumentation-tortoiseorm_ubuntu-latest:
+    name: instrumentation-tortoiseorm 3.8 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python 3.8
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.8"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e py38-test-instrumentation-tortoiseorm -- -ra
+
+  py39-test-instrumentation-tortoiseorm_ubuntu-latest:
+    name: instrumentation-tortoiseorm 3.9 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python 3.9
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.9"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e py39-test-instrumentation-tortoiseorm -- -ra
+
+  py310-test-instrumentation-tortoiseorm_ubuntu-latest:
+    name: instrumentation-tortoiseorm 3.10 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python 3.10
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.10"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e py310-test-instrumentation-tortoiseorm -- -ra
+
+  py311-test-instrumentation-tortoiseorm_ubuntu-latest:
+    name: instrumentation-tortoiseorm 3.11 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python 3.11
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.11"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e py311-test-instrumentation-tortoiseorm -- -ra
+
+  py312-test-instrumentation-tortoiseorm_ubuntu-latest:
+    name: instrumentation-tortoiseorm 3.12 Ubuntu
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python 3.12
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.12"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e py312-test-instrumentation-tortoiseorm -- -ra
+
   py313-test-instrumentation-tortoiseorm_ubuntu-latest:
     name: instrumentation-tortoiseorm 3.13 Ubuntu
     runs-on: ubuntu-latest
diff --git a/CHANGELOG.md b/CHANGELOG.md
index a8ebe71151..b1e78dc46c 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -29,6 +29,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   ([#3273](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3273))
 - Add `opentelemetry-instrumentation-vertexai>=2.0b0` to `opentelemetry-bootstrap`
   ([#3307](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3307))
+- Loosen `opentelemetry-instrumentation-starlette[instruments]` specifier
+  ([#3304](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/3304))
 
 
 ### Fixed
diff --git a/instrumentation/README.md b/instrumentation/README.md
index dcfcf46edd..5a87487773 100644
--- a/instrumentation/README.md
+++ b/instrumentation/README.md
@@ -43,7 +43,7 @@
 | [opentelemetry-instrumentation-requests](./opentelemetry-instrumentation-requests) | requests ~= 2.0 | Yes | migration
 | [opentelemetry-instrumentation-sqlalchemy](./opentelemetry-instrumentation-sqlalchemy) | sqlalchemy >= 1.0.0, < 2.1.0 | Yes | experimental
 | [opentelemetry-instrumentation-sqlite3](./opentelemetry-instrumentation-sqlite3) | sqlite3 | No | experimental
-| [opentelemetry-instrumentation-starlette](./opentelemetry-instrumentation-starlette) | starlette ~= 0.13.0 | Yes | experimental
+| [opentelemetry-instrumentation-starlette](./opentelemetry-instrumentation-starlette) | starlette >= 0.13, <0.15 | Yes | experimental
 | [opentelemetry-instrumentation-system-metrics](./opentelemetry-instrumentation-system-metrics) | psutil >= 5 | No | experimental
 | [opentelemetry-instrumentation-threading](./opentelemetry-instrumentation-threading) | threading | No | experimental
 | [opentelemetry-instrumentation-tornado](./opentelemetry-instrumentation-tornado) | tornado >= 5.1.1 | Yes | experimental
diff --git a/instrumentation/opentelemetry-instrumentation-starlette/pyproject.toml b/instrumentation/opentelemetry-instrumentation-starlette/pyproject.toml
index acb61760a1..102f245395 100644
--- a/instrumentation/opentelemetry-instrumentation-starlette/pyproject.toml
+++ b/instrumentation/opentelemetry-instrumentation-starlette/pyproject.toml
@@ -34,9 +34,7 @@ dependencies = [
 ]
 
 [project.optional-dependencies]
-instruments = [
-  "starlette ~= 0.13.0",
-]
+instruments = ["starlette >= 0.13, <0.15"]
 
 [project.entry-points.opentelemetry_instrumentor]
 starlette = "opentelemetry.instrumentation.starlette:StarletteInstrumentor"
@@ -49,10 +47,7 @@ Repository = "https://github.com/open-telemetry/opentelemetry-python-contrib"
 path = "src/opentelemetry/instrumentation/starlette/version.py"
 
 [tool.hatch.build.targets.sdist]
-include = [
-  "/src",
-  "/tests",
-]
+include = ["/src", "/tests"]
 
 [tool.hatch.build.targets.wheel]
 packages = ["src/opentelemetry"]
diff --git a/instrumentation/opentelemetry-instrumentation-starlette/src/opentelemetry/instrumentation/starlette/package.py b/instrumentation/opentelemetry-instrumentation-starlette/src/opentelemetry/instrumentation/starlette/package.py
index 11c3ed2e7b..ca95e754a8 100644
--- a/instrumentation/opentelemetry-instrumentation-starlette/src/opentelemetry/instrumentation/starlette/package.py
+++ b/instrumentation/opentelemetry-instrumentation-starlette/src/opentelemetry/instrumentation/starlette/package.py
@@ -13,6 +13,6 @@
 # limitations under the License.
 
 
-_instruments = ("starlette ~= 0.13.0",)
+_instruments = ("starlette >= 0.13, <0.15",)
 
 _supports_metrics = True
diff --git a/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in b/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
new file mode 100644
index 0000000000..15ff4892d5
--- /dev/null
+++ b/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
@@ -0,0 +1,8 @@
+# Used to generate test-requirements.{oldest,latest}.txt with uv pip compile
+pytest~=7.4
+requests~=2.32
+httpx~=0.28
+-e opentelemetry-instrumentation
+-e instrumentation/opentelemetry-instrumentation-asgi
+-e util/opentelemetry-util-http
+-e instrumentation/opentelemetry-instrumentation-starlette[instruments]
diff --git a/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.latest.txt b/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.latest.txt
new file mode 100644
index 0000000000..7b6dd8cff4
--- /dev/null
+++ b/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.latest.txt
@@ -0,0 +1,98 @@
+# This file was autogenerated by uv via the following command:
+#    uv pip compile --python 3.8 --universal --no-emit-package opentelemetry-api --no-emit-package opentelemetry-semantic-conventions -c dev-requirements.txt instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in -o instrumentation/opentelemetry-instrumentation-starlette/test-requirements.latest.txt
+-e instrumentation/opentelemetry-instrumentation-asgi
+    # via
+    #   -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+    #   opentelemetry-instrumentation-starlette
+-e instrumentation/opentelemetry-instrumentation-starlette
+    # via -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+-e opentelemetry-instrumentation
+    # via
+    #   -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+    #   opentelemetry-instrumentation-asgi
+    #   opentelemetry-instrumentation-starlette
+-e util/opentelemetry-util-http
+    # via
+    #   -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+    #   opentelemetry-instrumentation-asgi
+    #   opentelemetry-instrumentation-starlette
+anyio==4.5.2 ; python_full_version < '3.9'
+    # via httpx
+anyio==4.8.0 ; python_full_version >= '3.9'
+    # via httpx
+asgiref==3.8.1
+    # via opentelemetry-instrumentation-asgi
+certifi==2025.1.31
+    # via
+    #   httpcore
+    #   httpx
+    #   requests
+charset-normalizer==3.4.1
+    # via requests
+colorama==0.4.6 ; sys_platform == 'win32'
+    # via pytest
+deprecated==1.2.18
+    # via
+    #   opentelemetry-api
+    #   opentelemetry-semantic-conventions
+exceptiongroup==1.2.2 ; python_full_version < '3.11'
+    # via
+    #   anyio
+    #   pytest
+h11==0.14.0
+    # via httpcore
+httpcore==1.0.7
+    # via httpx
+httpx==0.28.1
+    # via -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+idna==3.10
+    # via
+    #   anyio
+    #   httpx
+    #   requests
+importlib-metadata==8.5.0 ; python_full_version < '3.9'
+    # via opentelemetry-api
+importlib-metadata==8.6.1 ; python_full_version >= '3.9'
+    # via opentelemetry-api
+iniconfig==2.0.0
+    # via pytest
+packaging==24.2
+    # via
+    #   opentelemetry-instrumentation
+    #   pytest
+pluggy==1.5.0
+    # via pytest
+pytest==7.4.4
+    # via
+    #   -c dev-requirements.txt
+    #   -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+requests==2.32.3
+    # via
+    #   -c dev-requirements.txt
+    #   -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+sniffio==1.3.1
+    # via anyio
+starlette==0.14.2
+    # via opentelemetry-instrumentation-starlette
+tomli==2.2.1 ; python_full_version < '3.11'
+    # via pytest
+typing-extensions==4.12.2 ; python_full_version < '3.13'
+    # via
+    #   anyio
+    #   asgiref
+urllib3==2.2.3 ; python_full_version < '3.9'
+    # via requests
+urllib3==2.3.0 ; python_full_version >= '3.9'
+    # via requests
+wrapt==1.17.2
+    # via
+    #   deprecated
+    #   opentelemetry-instrumentation
+zipp==3.20.2 ; python_full_version < '3.9'
+    # via importlib-metadata
+zipp==3.21.0 ; python_full_version >= '3.9'
+    # via importlib-metadata
+
+# The following packages were excluded from the output:
+# opentelemetry-api
+# opentelemetry-semantic-conventions
diff --git a/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.oldest.txt b/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.oldest.txt
new file mode 100644
index 0000000000..5f84cdf28f
--- /dev/null
+++ b/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.oldest.txt
@@ -0,0 +1,98 @@
+# This file was autogenerated by uv via the following command:
+#    uv pip compile --python 3.8 --universal --resolution lowest-direct --no-emit-package opentelemetry-api --no-emit-package opentelemetry-semantic-conventions -c dev-requirements.txt instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in -o instrumentation/opentelemetry-instrumentation-starlette/test-requirements.oldest.txt
+-e instrumentation/opentelemetry-instrumentation-asgi
+    # via
+    #   -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+    #   opentelemetry-instrumentation-starlette
+-e instrumentation/opentelemetry-instrumentation-starlette
+    # via -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+-e opentelemetry-instrumentation
+    # via
+    #   -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+    #   opentelemetry-instrumentation-asgi
+    #   opentelemetry-instrumentation-starlette
+-e util/opentelemetry-util-http
+    # via
+    #   -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+    #   opentelemetry-instrumentation-asgi
+    #   opentelemetry-instrumentation-starlette
+anyio==4.5.2 ; python_full_version < '3.9'
+    # via httpx
+anyio==4.8.0 ; python_full_version >= '3.9'
+    # via httpx
+asgiref==3.8.1
+    # via opentelemetry-instrumentation-asgi
+certifi==2025.1.31
+    # via
+    #   httpcore
+    #   httpx
+    #   requests
+charset-normalizer==3.4.1
+    # via requests
+colorama==0.4.6 ; sys_platform == 'win32'
+    # via pytest
+deprecated==1.2.14
+    # via
+    #   opentelemetry-api
+    #   opentelemetry-semantic-conventions
+exceptiongroup==1.2.2 ; python_full_version < '3.11'
+    # via
+    #   anyio
+    #   pytest
+h11==0.14.0
+    # via httpcore
+httpcore==1.0.7
+    # via httpx
+httpx==0.28.0
+    # via -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+idna==3.10
+    # via
+    #   anyio
+    #   httpx
+    #   requests
+importlib-metadata==8.5.0 ; python_full_version < '3.9'
+    # via opentelemetry-api
+importlib-metadata==8.6.1 ; python_full_version >= '3.9'
+    # via opentelemetry-api
+iniconfig==2.0.0
+    # via pytest
+packaging==24.0
+    # via
+    #   opentelemetry-instrumentation
+    #   pytest
+pluggy==1.5.0
+    # via pytest
+pytest==7.4.4
+    # via
+    #   -c dev-requirements.txt
+    #   -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+requests==2.32.3
+    # via
+    #   -c dev-requirements.txt
+    #   -r instrumentation/opentelemetry-instrumentation-starlette/test-requirements.in
+sniffio==1.3.1
+    # via anyio
+starlette==0.13.8
+    # via opentelemetry-instrumentation-starlette
+tomli==2.2.1 ; python_full_version < '3.11'
+    # via pytest
+typing-extensions==4.12.2 ; python_full_version < '3.13'
+    # via
+    #   anyio
+    #   asgiref
+urllib3==2.2.3 ; python_full_version < '3.9'
+    # via requests
+urllib3==2.3.0 ; python_full_version >= '3.9'
+    # via requests
+wrapt==1.16.0
+    # via
+    #   deprecated
+    #   opentelemetry-instrumentation
+zipp==3.20.2 ; python_full_version < '3.9'
+    # via importlib-metadata
+zipp==3.21.0 ; python_full_version >= '3.9'
+    # via importlib-metadata
+
+# The following packages were excluded from the output:
+# opentelemetry-api
+# opentelemetry-semantic-conventions
diff --git a/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.txt
deleted file mode 100644
index 3d756aa52e..0000000000
--- a/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.txt
+++ /dev/null
@@ -1,27 +0,0 @@
-anyio==4.3.0
-asgiref==3.8.1
-certifi==2024.7.4
-charset-normalizer==3.3.2
-Deprecated==1.2.14
-exceptiongroup==1.2.0
-h11==0.14.0
-httpcore==1.0.4
-httpx==0.27.0
-idna==3.7
-iniconfig==2.0.0
-packaging==24.0
-pluggy==1.5.0
-py-cpuinfo==9.0.0
-pytest==7.4.4
-requests==2.32.3
-sniffio==1.3.0
-starlette==0.13.8
-tomli==2.0.1
-typing_extensions==4.12.2
-urllib3==2.2.2
-wrapt==1.16.0
-zipp==3.19.2
--e opentelemetry-instrumentation
--e instrumentation/opentelemetry-instrumentation-asgi
--e util/opentelemetry-util-http
--e instrumentation/opentelemetry-instrumentation-starlette
diff --git a/instrumentation/opentelemetry-instrumentation-threading/src/opentelemetry/instrumentation/threading/__init__.py b/instrumentation/opentelemetry-instrumentation-threading/src/opentelemetry/instrumentation/threading/__init__.py
index 18604d8596..0befa26165 100644
--- a/instrumentation/opentelemetry-instrumentation-threading/src/opentelemetry/instrumentation/threading/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-threading/src/opentelemetry/instrumentation/threading/__init__.py
@@ -150,7 +150,7 @@ def __wrap_threading_run(
             token = context.attach(instance._otel_context)
             return call_wrapped(*args, **kwargs)
         finally:
-            context.detach(token)
+            context.detach(token)  # type: ignore[reportArgumentType] remove with https://github.com/open-telemetry/opentelemetry-python-contrib/issues/3321
 
     @staticmethod
     def __wrap_thread_pool_submit(
@@ -169,7 +169,7 @@ def wrapped_func(*func_args: Any, **func_kwargs: Any) -> R:
                 token = context.attach(otel_context)
                 return original_func(*func_args, **func_kwargs)
             finally:
-                context.detach(token)
+                context.detach(token)  # type: ignore[reportArgumentType] remove with https://github.com/open-telemetry/opentelemetry-python-contrib/issues/3321
 
         # replace the original function with the wrapped function
         new_args: tuple[Callable[..., Any], ...] = (wrapped_func,) + args[1:]
diff --git a/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap_gen.py b/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap_gen.py
index 78dd70faa1..8162081932 100644
--- a/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap_gen.py
+++ b/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap_gen.py
@@ -185,7 +185,7 @@
         "instrumentation": "opentelemetry-instrumentation-sqlalchemy==0.52b0.dev",
     },
     {
-        "library": "starlette ~= 0.13.0",
+        "library": "starlette >= 0.13, <0.15",
         "instrumentation": "opentelemetry-instrumentation-starlette==0.52b0.dev",
     },
     {
diff --git a/pyproject.toml b/pyproject.toml
index 89f415d77c..f58229522f 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -144,7 +144,7 @@ members = [
   "propagator/*",
   "util/opentelemetry-util-http",
 ]
-# TODO: remove after https://github.com/open-telemetry/opentelemetry-python-contrib/issues/3300
+# TODO: remove after python 3.8 is dropped
 exclude = [
   "instrumentation-genai/opentelemetry-instrumentation-google-genai",
 ]
diff --git a/tox.ini b/tox.ini
index 6f74535ae4..afcc054e0f 100644
--- a/tox.ini
+++ b/tox.ini
@@ -175,8 +175,8 @@ envlist =
     lint-instrumentation-requests
 
     ; opentelemetry-instrumentation-starlette
-    py3{8,9,10,11,12,13}-test-instrumentation-starlette
-    pypy3-test-instrumentation-starlette
+    py3{8,9,10,11,12,13}-test-instrumentation-starlette-{oldest,latest}
+    pypy3-test-instrumentation-starlette-{oldest,latest}
     lint-instrumentation-starlette
 
     ; opentelemetry-instrumentation-jinja2
@@ -612,7 +612,8 @@ deps =
   requests: -r {toxinidir}/instrumentation/opentelemetry-instrumentation-requests/test-requirements.txt
 
   starlette: {[testenv]test_deps}
-  starlette: -r {toxinidir}/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.txt
+  starlette-{oldest,lint}: -r {toxinidir}/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.oldest.txt
+  starlette-latest: -r {toxinidir}/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.latest.txt
 
   system-metrics: {[testenv]test_deps}
   system-metrics: -r {toxinidir}/instrumentation/opentelemetry-instrumentation-system-metrics/test-requirements.txt
diff --git a/uv.lock b/uv.lock
index 11487f761e..c2b160252f 100644
--- a/uv.lock
+++ b/uv.lock
@@ -3773,7 +3773,7 @@ requires-dist = [
     { name = "opentelemetry-instrumentation-asgi", editable = "instrumentation/opentelemetry-instrumentation-asgi" },
     { name = "opentelemetry-semantic-conventions", git = "https://github.com/open-telemetry/opentelemetry-python?subdirectory=opentelemetry-semantic-conventions&branch=main" },
     { name = "opentelemetry-util-http", editable = "util/opentelemetry-util-http" },
-    { name = "starlette", marker = "extra == 'instruments'", specifier = "~=0.13.0" },
+    { name = "starlette", marker = "extra == 'instruments'", specifier = ">=0.13,<0.15" },
 ]
 provides-extras = ["instruments"]