cellranger multi with gene expression and CRISPR data fails to process CRISPR data #7174
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bug
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Description of the bug
Running the pipeline using
cellranger multi
(cellrangermulti
aligner option) with gene expression and CRISPR libraries (e.g., this dataset) fails to process CRISPR data (only gene expression data are processed). This could be traced to the following:modules/nf-core/cellranger/multi/templates/cellranger_multi.py
:for modality in ["gex", "vdj", "ab", "beam", "cmo", "cirspr"]:
to be replaced byfor modality in ["gex", "vdj", "ab", "beam", "cmo", "crispr"]:
modules/nf-core/cellranger/multi/main.nf
:include_fb = ab_fastqs.first().getName() != 'fastqs' && fb_reference ? '[feature]' : ''
to be replaced byinclude_fb = (ab_fastqs.first().getName() != 'fastqs' || crispr_fastqs.first().getName() != 'fastqs') && fb_reference ? '[feature]' : ''
I will submit a pull request with the fixes.
Command used and terminal output
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