From a230b43fb6b3207580b0b9d1dedd6d7b333b37a7 Mon Sep 17 00:00:00 2001 From: Nils Homer Date: Thu, 23 May 2024 17:08:00 -0400 Subject: [PATCH 01/30] updates post 1.0.0 release --- CHANGELOG.md | 2 ++ README.md | 5 +---- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- tests/pipeline/multi_fastq.nf.test.snap | 6 +++--- tests/pipeline/multi_lanes.nf.test.snap | 6 +++--- tests/pipeline/single_fastq.nf.test.snap | 6 +++--- tests/pipeline/tiny.nf.test.snap | 6 +++--- 8 files changed, 18 insertions(+), 19 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 21b5367..5db8f75 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,8 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). +## [Unpublished Version / DEV] + ## [[1.0.0]](https://github.com/nf-core/fastquorum/releases/tag/1.0.0)] -- 2024-05-20 Initial release of nf-core/fastquorum, created with the [nf-core](https://nf-co.re/) template. diff --git a/README.md b/README.md index 96178d7..ca743ff 100644 --- a/README.md +++ b/README.md @@ -152,10 +152,7 @@ For further information or help, don't hesitate to get in touch on the [Slack `# ## Citations -If you use nf-core/fastquorum for your analysis, please cite for this pipeline and [![DOI](https://zenodo.org/badge/53011104.svg)](https://zenodo.org/doi/10.5281/zenodo.10456900) for [`fgbio`](https://github.com/fulcrumgenomics/fgbio). - - - +If you use nf-core/fastquorum for your analysis, please cite [![DOI](https://zenodo.org/badge/DOI/10.5281/zenodo.11267673.svg)](https://doi.org/10.5281/zenodo.11267673) for this pipeline and [![DOI](https://zenodo.org/badge/53011104.svg)](https://zenodo.org/doi/10.5281/zenodo.10456900) for [`fgbio`](https://github.com/fulcrumgenomics/fgbio). An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 57fb11b..c2b23db 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/fastquorum + This report has been generated by the nf-core/fastquorum analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-fastquorum-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index bbf3af6..d68f446 100644 --- a/nextflow.config +++ b/nextflow.config @@ -254,7 +254,7 @@ manifest { description = """fgbio Best Practices FASTQ to Consensus Pipeline""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.0.0' + version = '1.0.1dev' doi = '10.5281/zenodo.10456900' } diff --git a/tests/pipeline/multi_fastq.nf.test.snap b/tests/pipeline/multi_fastq.nf.test.snap index c6b95fc..2be36f0 100644 --- a/tests/pipeline/multi_fastq.nf.test.snap +++ b/tests/pipeline/multi_fastq.nf.test.snap @@ -16,7 +16,7 @@ }, "multi_fastq_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.0}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", @@ -26,7 +26,7 @@ }, "multi_fastq_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.0}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", @@ -49,4 +49,4 @@ }, "timestamp": "2024-05-20T01:58:10.241449" } -} \ No newline at end of file +} diff --git a/tests/pipeline/multi_lanes.nf.test.snap b/tests/pipeline/multi_lanes.nf.test.snap index 43f42c5..120754e 100644 --- a/tests/pipeline/multi_lanes.nf.test.snap +++ b/tests/pipeline/multi_lanes.nf.test.snap @@ -1,7 +1,7 @@ { "multi_lanes_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, Workflow={nf-core/fastquorum=v1.0.0}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", @@ -26,7 +26,7 @@ }, "multi_lanes_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, Workflow={nf-core/fastquorum=v1.0.0}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", @@ -49,4 +49,4 @@ }, "timestamp": "2024-05-20T01:34:44.993842" } -} \ No newline at end of file +} diff --git a/tests/pipeline/single_fastq.nf.test.snap b/tests/pipeline/single_fastq.nf.test.snap index 110e78e..36164c2 100644 --- a/tests/pipeline/single_fastq.nf.test.snap +++ b/tests/pipeline/single_fastq.nf.test.snap @@ -11,7 +11,7 @@ }, "single_fastq_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.0}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", @@ -21,7 +21,7 @@ }, "single_fastq_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.0}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", @@ -39,4 +39,4 @@ }, "timestamp": "2024-05-20T07:39:38.166122" } -} \ No newline at end of file +} diff --git a/tests/pipeline/tiny.nf.test.snap b/tests/pipeline/tiny.nf.test.snap index 72b51a6..e2f8356 100644 --- a/tests/pipeline/tiny.nf.test.snap +++ b/tests/pipeline/tiny.nf.test.snap @@ -1,7 +1,7 @@ { "tiny_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.0}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", @@ -11,7 +11,7 @@ }, "tiny_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.0}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", @@ -41,4 +41,4 @@ }, "timestamp": "2024-05-19T23:48:57.112733" } -} \ No newline at end of file +} From a60b8249088ab4755154dfbb33b206110fab152b Mon Sep 17 00:00:00 2001 From: Nils Homer Date: Thu, 23 May 2024 21:17:38 -0400 Subject: [PATCH 02/30] Update README.md --- README.md | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/README.md b/README.md index ca743ff..0d0001a 100644 --- a/README.md +++ b/README.md @@ -6,9 +6,8 @@ [![GitHub Actions CI Status](https://github.com/nf-core/fastquorum/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/fastquorum/actions/workflows/ci.yml) -[![GitHub Actions Linting Status](https://github.com/nf-core/fastquorum/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/fastquorum/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/fastquorum/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![GitHub Actions Linting Status](https://github.com/nf-core/fastquorum/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/fastquorum/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/fastquorum/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.11267673-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.11267673) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) - [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) From 556e5f744fdb475244345742ae87002e43d26814 Mon Sep 17 00:00:00 2001 From: Nils Homer Date: Sat, 25 May 2024 15:41:25 -0400 Subject: [PATCH 03/30] ci: fix nf-test fastquorum versions for v1.0.1 --- tests/pipeline/multi_fastq.nf.test.snap | 4 ++-- tests/pipeline/multi_lanes.nf.test.snap | 4 ++-- tests/pipeline/single_fastq.nf.test.snap | 4 ++-- tests/pipeline/tiny.nf.test.snap | 4 ++-- 4 files changed, 8 insertions(+), 8 deletions(-) diff --git a/tests/pipeline/multi_fastq.nf.test.snap b/tests/pipeline/multi_fastq.nf.test.snap index 2be36f0..06c4533 100644 --- a/tests/pipeline/multi_fastq.nf.test.snap +++ b/tests/pipeline/multi_fastq.nf.test.snap @@ -16,7 +16,7 @@ }, "multi_fastq_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", @@ -26,7 +26,7 @@ }, "multi_fastq_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", diff --git a/tests/pipeline/multi_lanes.nf.test.snap b/tests/pipeline/multi_lanes.nf.test.snap index 120754e..d48b1b3 100644 --- a/tests/pipeline/multi_lanes.nf.test.snap +++ b/tests/pipeline/multi_lanes.nf.test.snap @@ -1,7 +1,7 @@ { "multi_lanes_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", @@ -26,7 +26,7 @@ }, "multi_lanes_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", diff --git a/tests/pipeline/single_fastq.nf.test.snap b/tests/pipeline/single_fastq.nf.test.snap index 36164c2..9a82518 100644 --- a/tests/pipeline/single_fastq.nf.test.snap +++ b/tests/pipeline/single_fastq.nf.test.snap @@ -11,7 +11,7 @@ }, "single_fastq_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", @@ -21,7 +21,7 @@ }, "single_fastq_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", diff --git a/tests/pipeline/tiny.nf.test.snap b/tests/pipeline/tiny.nf.test.snap index e2f8356..32dcb04 100644 --- a/tests/pipeline/tiny.nf.test.snap +++ b/tests/pipeline/tiny.nf.test.snap @@ -1,7 +1,7 @@ { "tiny_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", @@ -11,7 +11,7 @@ }, "tiny_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", From d8fa98561d07b068a4039d986837415800d8f4c6 Mon Sep 17 00:00:00 2001 From: Nils Homer Date: Mon, 27 May 2024 13:05:19 -0400 Subject: [PATCH 04/30] Apply suggestions from code review Co-authored-by: Maxime U Garcia --- README.md | 4 ++-- nextflow.config | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/README.md b/README.md index 0d0001a..a90dfc2 100644 --- a/README.md +++ b/README.md @@ -6,7 +6,7 @@ [![GitHub Actions CI Status](https://github.com/nf-core/fastquorum/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/fastquorum/actions/workflows/ci.yml) -[![GitHub Actions Linting Status](https://github.com/nf-core/fastquorum/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/fastquorum/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/fastquorum/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.11267673-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.11267673) +[![GitHub Actions Linting Status](https://github.com/nf-core/fastquorum/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/fastquorum/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/fastquorum/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.11267672-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.11267672) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) @@ -151,7 +151,7 @@ For further information or help, don't hesitate to get in touch on the [Slack `# ## Citations -If you use nf-core/fastquorum for your analysis, please cite [![DOI](https://zenodo.org/badge/DOI/10.5281/zenodo.11267673.svg)](https://doi.org/10.5281/zenodo.11267673) for this pipeline and [![DOI](https://zenodo.org/badge/53011104.svg)](https://zenodo.org/doi/10.5281/zenodo.10456900) for [`fgbio`](https://github.com/fulcrumgenomics/fgbio). +If you use nf-core/fastquorum for your analysis, please cite [![DOI](https://zenodo.org/badge/DOI/10.5281/zenodo.11267672.svg)](https://doi.org/10.5281/zenodo.11267672) for this pipeline and [![DOI](https://zenodo.org/badge/53011104.svg)](https://zenodo.org/doi/10.5281/zenodo.10456900) for [`fgbio`](https://github.com/fulcrumgenomics/fgbio). An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. diff --git a/nextflow.config b/nextflow.config index d68f446..0a6fe3f 100644 --- a/nextflow.config +++ b/nextflow.config @@ -255,7 +255,7 @@ manifest { mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' version = '1.0.1dev' - doi = '10.5281/zenodo.10456900' + doi = '10.5281/zenodo.10456900,10.5281/zenodo.11267672' } // Load modules.config for DSL2 module specific options From 0f722764d3839577a4e2b391a9b11c39519ba3f4 Mon Sep 17 00:00:00 2001 From: Nils Homer Date: Sat, 25 May 2024 15:37:36 -0400 Subject: [PATCH 05/30] docs: update subway diagrams Fixes a bug where alignment and filtering where swapped in the phase 2 high-throughput diagrams. Thanks James Fellows Yates (@jfy133) --- docs/images/fastquorum_subway.desc.png | Bin 361579 -> 361652 bytes docs/images/fastquorum_subway.desc.svg | 594 ++++++++++++------------ docs/images/fastquorum_subway.png | Bin 441671 -> 441608 bytes docs/images/fastquorum_subway.svg | 610 ++++++++++++------------- 4 files changed, 599 insertions(+), 605 deletions(-) diff --git a/docs/images/fastquorum_subway.desc.png b/docs/images/fastquorum_subway.desc.png index f0562b977b510129e65b0e4ca459b07eac5ba959..3e3dc7856be8a3b0075cf7cf597f983de4649377 100644 GIT binary patch delta 281981 zcma%jcRZKv`#vd^h=!8YPEk=PTLXoVEksgTSs7*CEu(}=C^E9Q6xk%JWJLB1WzWpa z-*NXmJ2up2u;V$9WIBZ%KWy<#sCNY0eAr4lHA$yCv-v zSFYsOa4%_B+>qVKA7gm%$n>0){@N1%qAmIlr1Xa#tXkK|qo{aq6@S=I8jZ^ZE4G~2 z@OYP`(8S^s1%kQPE{eW*UehD3YshK3iI&&rL#3eqdM-*^|0+&OOM-fD8^wnI^LirU z;^Hzp^tNiOuR46@^4BQ2!mYZFua=|Eny+USxc%d^fBwv^A7#FlW8Y3b4-E7_p{~+w zxx>r-|GZ=(?IwCUzZKjY6*aql{SmvU{j(EiA{2sygPFvgMRf8Uf9YH&7G}rStXZ@F 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From 2bb38cf398d844a5e9af0f01bee30021e8b7b563 Mon Sep 17 00:00:00 2001 From: Simon Pearce <24893913+SPPearce@users.noreply.github.com> Date: Wed, 5 Jun 2024 14:50:45 +0000 Subject: [PATCH 07/30] Add when conditions to prepare genome so they only run if not given --- conf/modules.config | 21 ++++++++++++++++++++- 1 file changed, 20 insertions(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 6e53bc8..bd8baf4 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,7 +18,26 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: 'BWA_INDEX|SAMTOOLS_FAIDX|SAMTOOLS_DICT' { + withName: 'BWA_INDEX' { + ext.when = { !params.bwa } + publishDir = [ + path: { params.save_reference ? "${params.outdir}/genome" : params.outdir }, + mode: params.publish_dir_mode, + saveAs: { filename -> (filename != 'versions.yml' && params.save_reference) ? filename : null } + ] + } + + withName: 'SAMTOOLS_FAIDX' { + ext.when = { !params.fasta_fai } + publishDir = [ + path: { params.save_reference ? "${params.outdir}/genome" : params.outdir }, + mode: params.publish_dir_mode, + saveAs: { filename -> (filename != 'versions.yml' && params.save_reference) ? filename : null } + ] + } + + withName: 'SAMTOOLS_DICT' { + ext.when = { !params.dict } publishDir = [ path: { params.save_reference ? "${params.outdir}/genome" : params.outdir }, mode: params.publish_dir_mode, From 916a1bf1e85f8d2232b2063c25592f0bce1dee09 Mon Sep 17 00:00:00 2001 From: Simon Pearce <24893913+SPPearce@users.noreply.github.com> Date: Wed, 5 Jun 2024 14:54:40 +0000 Subject: [PATCH 08/30] Update Changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 47dbde9..ebec627 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,6 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Enhancements & fixes - [PR #51](https://github.com/nf-core/fastquorum/pull/51) - Fixes a bug where alignment and filtering where swapped in the phase 2 high-throughput diagrams (@jfy133). +- [PR #58](https://github.com/nf-core/fastquorum/pull/58) - Prepare genome steps now run only if the corresponding parameters are not passed. ## [[1.0.0]](https://github.com/nf-core/fastquorum/releases/tag/1.0.0)] -- 2024-05-20 From 4e136c611ff23475cb248324f0abbd826b7de2ba Mon Sep 17 00:00:00 2001 From: Simon Pearce <24893913+SPPearce@users.noreply.github.com> Date: Mon, 15 Jul 2024 14:05:25 +0000 Subject: [PATCH 09/30] Move filterconsensusreads memory to base.config --- conf/base.config | 8 +++----- conf/modules.config | 4 ---- 2 files changed, 3 insertions(+), 9 deletions(-) diff --git a/conf/base.config b/conf/base.config index b517b57..655da65 100644 --- a/conf/base.config +++ b/conf/base.config @@ -21,11 +21,6 @@ process { maxErrors = '-1' // Process-specific resource requirements - // NOTE - Please try and re-use the labels below as much as possible. - // These labels are used and recognised by default in DSL2 files hosted on nf-core/modules. - // If possible, it would be nice to keep the same label naming convention when - // adding in your local modules too. - // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors withLabel:process_single { cpus = { check_max( 1 , 'cpus' ) } memory = { check_max( 6.GB * task.attempt, 'memory' ) } @@ -67,4 +62,7 @@ process { cpus = { check_max( 4 * task.attempt, 'cpus' ) } memory = { check_max( 12.GB * task.attempt, 'memory' ) } } + withName:'FILTERCONSENSUSREADS' { + memory = { check_max( 12.GB * task.attempt, 'memory' ) } + } } diff --git a/conf/modules.config b/conf/modules.config index 6e53bc8..ae21a9e 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -107,10 +107,6 @@ process { ] } - withName: '.*FILTERCONSENSUSREADS' { - memory = { check_max( 8.GB * task.attempt, 'memory' ) } - } - withName: '.*CALLANDFILTERDUPLEXCONSENSUSREADS|.*CALLANDFILTERMOLECULARCONSENSUSREADS|.*FILTERCONSENSUSREADS' { publishDir = [ [ From 20f3ce0663db8f74f2094250bb53ae0aa93e4f78 Mon Sep 17 00:00:00 2001 From: Simon Pearce <24893913+SPPearce@users.noreply.github.com> Date: Mon, 15 Jul 2024 14:07:32 +0000 Subject: [PATCH 10/30] Update Changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 47dbde9..7e59eaa 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,6 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Enhancements & fixes - [PR #51](https://github.com/nf-core/fastquorum/pull/51) - Fixes a bug where alignment and filtering where swapped in the phase 2 high-throughput diagrams (@jfy133). +- [PR #60](https://github.com/nf-core/fastquorum/pull/60) - Enable automatic escalation of memory for FilterConsensusReads. ## [[1.0.0]](https://github.com/nf-core/fastquorum/releases/tag/1.0.0)] -- 2024-05-20 From af43d9fde47e0c35808a15423dacbe99acb0cde4 Mon Sep 17 00:00:00 2001 From: Simon Pearce <24893913+SPPearce@users.noreply.github.com> Date: Thu, 18 Jul 2024 09:25:26 +0000 Subject: [PATCH 11/30] Move process resource requests into modules.config --- conf/base.config | 11 ----------- conf/modules.config | 9 ++++++++- 2 files changed, 8 insertions(+), 12 deletions(-) diff --git a/conf/base.config b/conf/base.config index 655da65..621a236 100644 --- a/conf/base.config +++ b/conf/base.config @@ -54,15 +54,4 @@ process { errorStrategy = 'retry' maxRetries = 2 } - withName:'FASTQC'{ - cpus = { check_max( 4 * task.attempt, 'cpus' ) } - memory = { check_max( 4.GB * task.attempt, 'memory' ) } - } - withName:'MULTIQC' { - cpus = { check_max( 4 * task.attempt, 'cpus' ) } - memory = { check_max( 12.GB * task.attempt, 'memory' ) } - } - withName:'FILTERCONSENSUSREADS' { - memory = { check_max( 12.GB * task.attempt, 'memory' ) } - } } diff --git a/conf/modules.config b/conf/modules.config index 8a8fb01..3bc0231 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -46,6 +46,8 @@ process { } withName: 'FASTQC' { + cpus = { check_max( 4 * task.attempt, 'cpus' ) } + memory = { check_max( 4.GB * task.attempt, 'memory' ) } ext.args = '--quiet' publishDir = [ path: { "${params.outdir}/preprocessing/fastqc/${meta.id}" }, @@ -141,6 +143,10 @@ process { ] } + withName:'FILTERCONSENSUSREADS' { + memory = { check_max( 12.GB * task.attempt, 'memory' ) } + } + withName: '.*ALIGN_CONSENSUS_BAM' { publishDir = [ path: { "${params.outdir}/filtering/align_consensus_bam/${meta.id}" }, @@ -150,6 +156,8 @@ process { } withName: 'MULTIQC' { + cpus = { check_max( 4 * task.attempt, 'cpus' ) } + memory = { check_max( 12.GB * task.attempt, 'memory' ) } ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ path: { "${params.outdir}/multiqc" }, @@ -158,5 +166,4 @@ process { ] } - } From 0c09e781b0fadf053d0d47e46704dd358b06092d Mon Sep 17 00:00:00 2001 From: Simon Pearce <24893913+SPPearce@users.noreply.github.com> Date: Fri, 19 Jul 2024 12:02:51 +0000 Subject: [PATCH 12/30] Allow igenomes to set params --- conf/igenomes.config | 4 ++++ main.nf | 21 ++++++++++----------- nextflow.config | 8 ++------ 3 files changed, 16 insertions(+), 17 deletions(-) diff --git a/conf/igenomes.config b/conf/igenomes.config index 3f11437..cd3a6d3 100644 --- a/conf/igenomes.config +++ b/conf/igenomes.config @@ -13,6 +13,8 @@ params { genomes { 'GRCh37' { fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" + fasta_fai = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa.fai" + dict = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.dict" bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/version0.6.0/" bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" @@ -26,6 +28,8 @@ params { } 'GRCh38' { fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" + fasta_fai = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa.fai" + dict = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.dict" bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/version0.6.0/" bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" diff --git a/main.nf b/main.nf index 42cdbd9..ae79a22 100644 --- a/main.nf +++ b/main.nf @@ -13,27 +13,26 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS + GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ - -include { FASTQUORUM } from './workflows/fastquorum' -include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_fastquorum_pipeline' -include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_fastquorum_pipeline' include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_fastquorum_pipeline' -include { PREPARE_GENOME } from './subworkflows/local/prepare_genome' +params.fasta = getGenomeAttribute('fasta') +params.fasta_fai = getGenomeAttribute('fasta_fai') +params.dict = getGenomeAttribute('dict') +params.bwa = getGenomeAttribute('bwa') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -params.fasta = getGenomeAttribute('fasta') -params.fasta_fai = getGenomeAttribute('fasta_fai') -params.dict = getGenomeAttribute('dict') -params.bwa = getGenomeAttribute('bwa') +include { FASTQUORUM } from './workflows/fastquorum' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_fastquorum_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_fastquorum_pipeline' +include { PREPARE_GENOME } from './subworkflows/local/prepare_genome' // Initialize fasta file with meta map: fasta = params.fasta ? Channel.fromPath(params.fasta).map{ it -> [ [id:it.baseName], it ] }.collect() : Channel.empty() diff --git a/nextflow.config b/nextflow.config index 0a6fe3f..37721fc 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,10 +16,6 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - fasta = null - bwa = null - dict = null - fasta_fai = null save_reference = false // MultiQC options @@ -73,8 +69,8 @@ params { // Schema validation default options validationFailUnrecognisedParams = false - validationLenientMode = false - validationSchemaIgnoreParams = 'genomes,igenomes_base,monochromeLogs' + validationLenientMode = true + validationSchemaIgnoreParams = 'genomes,igenomes_base,monochromeLogs,fasta,fasta_fai,bwa,dict' validationShowHiddenParams = false validate_params = true } From 962475bbe5128cbaa7416e0b833db0bfab166f7f Mon Sep 17 00:00:00 2001 From: Nils Homer Date: Fri, 19 Jul 2024 15:20:38 -0400 Subject: [PATCH 13/30] fix: support when multiple value args can be a number or a comma-delimited string Fixes #62 --- nextflow_schema.json | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index d7c2c51..c0bb332 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -91,7 +91,7 @@ "description": "Options for when creating consensus reads", "properties": { "call_min_reads": { - "type": "string", + "type": ["integer", "string"], "description": "Minimum reads to call a consensus", "fa_icon": "fas fa-book", "help_text": "The minimum reads to call a consensus for fgbio's CallMolecularConsensusReads/CallDuplexConsensusReads." @@ -111,7 +111,7 @@ "description": "Options for when filtering consensus reads", "properties": { "filter_min_reads": { - "type": "string", + "type": ["integer", "string"], "description": "Minimum reads to keep a consensus", "fa_icon": "fas fa-book", "help_text": "The minimum reads to keep a consensus for fgbio's FilterConsensusReads." @@ -123,7 +123,7 @@ "help_text": "The minimum consensus base quality to keep when calling a consensus for fgbio's FilterConsensusReads." }, "filter_max_base_error_rate": { - "type": "number", + "type": ["number", "string"], "description": "The maximum error rate for a single consensus base", "fa_icon": "fas fa-book", "help_text": "The maximum error rate for a single consensus base when filtering a consensus for fgbio's FilterConsensusReads." From 11efc40637a462c412e1533b1b2b7c4b519d8534 Mon Sep 17 00:00:00 2001 From: Simon Pearce <24893913+SPPearce@users.noreply.github.com> Date: Mon, 22 Jul 2024 14:44:47 +0000 Subject: [PATCH 14/30] Remove uncalled checkPathParamList --- .../local/utils_nfcore_fastquorum_pipeline/main.nf | 9 --------- 1 file changed, 9 deletions(-) diff --git a/subworkflows/local/utils_nfcore_fastquorum_pipeline/main.nf b/subworkflows/local/utils_nfcore_fastquorum_pipeline/main.nf index 3c8f5c3..d9e4490 100644 --- a/subworkflows/local/utils_nfcore_fastquorum_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_fastquorum_pipeline/main.nf @@ -77,15 +77,6 @@ workflow PIPELINE_INITIALISATION { // validateInputParameters() - // Check input path parameters to see if they exist - def checkPathParamList = [ - params.bwa, - params.dict, - params.fasta, - params.fasta_fai - - ] - // // Create channel from input file provided through params.input // From 1f49d6bcaede700de65c4ef6611bcd18020f3ec4 Mon Sep 17 00:00:00 2001 From: Simon Pearce <24893913+SPPearce@users.noreply.github.com> Date: Mon, 22 Jul 2024 15:23:26 +0000 Subject: [PATCH 15/30] Update CHANGELOG and schema --- CHANGELOG.md | 1 + nextflow_schema.json | 3 +++ 2 files changed, 4 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 8d8eb8f..c3687ce 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,6 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [PR #51](https://github.com/nf-core/fastquorum/pull/51) - Fixes a bug where alignment and filtering where swapped in the phase 2 high-throughput diagrams (@jfy133). - [PR #58](https://github.com/nf-core/fastquorum/pull/58) - Prepare genome steps now run only if the corresponding parameters are not passed. - [PR #60](https://github.com/nf-core/fastquorum/pull/60) - Enable automatic escalation of memory for FilterConsensusReads. +- [PR #67](https://github.com/nf-core/fastquorum/pull/67) - Fix setting the parameters from igenomes. ## [[1.0.0]](https://github.com/nf-core/fastquorum/releases/tag/1.0.0)] -- 2024-05-20 diff --git a/nextflow_schema.json b/nextflow_schema.json index d7c2c51..29e0a90 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -155,6 +155,7 @@ "dict": { "type": "string", "fa_icon": "fas fa-file", + "exists": true, "description": "Path to FASTA dictionary file.", "help_text": "If you use AWS iGenomes, this has already been set for you appropriately.\n\n> **NB** If none provided, will be generated automatically from the FASTA reference. Combine with `--save_reference` to save for future runs.", "hidden": true @@ -162,12 +163,14 @@ "fasta_fai": { "type": "string", "fa_icon": "fas fa-file", + "exists": true, "help_text": "If you use AWS iGenomes, this has already been set for you appropriately.\n\n> **NB** If none provided, will be generated automatically from the FASTA reference. Combine with `--save_reference` to save for future runs.", "description": "Path to FASTA reference index." }, "bwa": { "type": "string", "fa_icon": "fas fa-copy", + "exists": true, "description": "Path to BWA mem indices.", "help_text": "If you use AWS iGenomes, this has already been set for you appropriately.\n\nIf you wish to recompute indices available on igenomes, set `--bwa false`.\n\n> **NB** If none provided, will be generated automatically from the FASTA reference. Combine with `--save_reference` to save for future runs.", "hidden": true From fb728062f9f53b319e690ce7ec39735be5635140 Mon Sep 17 00:00:00 2001 From: Simon Pearce <24893913+SPPearce@users.noreply.github.com> Date: Thu, 1 Aug 2024 21:55:41 +0100 Subject: [PATCH 16/30] Update nextflow.config Co-authored-by: Nils Homer --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 37721fc..c7cc4fb 100644 --- a/nextflow.config +++ b/nextflow.config @@ -69,7 +69,7 @@ params { // Schema validation default options validationFailUnrecognisedParams = false - validationLenientMode = true + validationLenientMode = false validationSchemaIgnoreParams = 'genomes,igenomes_base,monochromeLogs,fasta,fasta_fai,bwa,dict' validationShowHiddenParams = false validate_params = true From b0e81a018be4cf44f8ce0618d3e904cd8e45624d Mon Sep 17 00:00:00 2001 From: Nils Homer Date: Thu, 30 May 2024 01:39:55 -0400 Subject: [PATCH 17/30] bugfix: merging lanes would result in an error There's a bug in `samtools merge --template-coordinate' that causes the output not to be correctly sorted. Here, we re-sort via fgbio SortBam to ensure the output is properly sorted. fgbio SortBam can be removed when the fix to the samtools bug is released. See: https://github.com/samtools/samtools/pull/2062 --- CHANGELOG.md | 4 ++ conf/modules.config | 11 +++++ modules.json | 5 +++ modules/nf-core/fgbio/sortbam/environment.yml | 7 ++++ modules/nf-core/fgbio/sortbam/main.nf | 36 ++++++++++++++++ modules/nf-core/fgbio/sortbam/meta.yml | 42 +++++++++++++++++++ tests/pipeline/multi_lanes.nf.test | 4 +- tests/pipeline/multi_lanes.nf.test.snap | 8 ++-- workflows/fastquorum.nf | 10 ++++- 9 files changed, 120 insertions(+), 7 deletions(-) create mode 100644 modules/nf-core/fgbio/sortbam/environment.yml create mode 100644 modules/nf-core/fgbio/sortbam/main.nf create mode 100644 modules/nf-core/fgbio/sortbam/meta.yml diff --git a/CHANGELOG.md b/CHANGELOG.md index 8d8eb8f..432a846 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -11,6 +11,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [PR #58](https://github.com/nf-core/fastquorum/pull/58) - Prepare genome steps now run only if the corresponding parameters are not passed. - [PR #60](https://github.com/nf-core/fastquorum/pull/60) - Enable automatic escalation of memory for FilterConsensusReads. +### Enhancements & fixes + +- [PR #54](https://github.com/nf-core/fastquorum/pull/54) - Temporary fix to merging BAMs across lanes in template-coordinate order. Using `fgbio SortBam` after `samtools merge`. Related to [samtools#2062](https://github.com/samtools/samtools/pull/2062). + ## [[1.0.0]](https://github.com/nf-core/fastquorum/releases/tag/1.0.0)] -- 2024-05-20 Initial release of nf-core/fastquorum, created with the [nf-core](https://nf-co.re/) template. diff --git a/conf/modules.config b/conf/modules.config index 3bc0231..6d908fb 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -88,6 +88,17 @@ process { ] } + /* This can be removed when https://github.com/samtools/samtools/pull/2062 is merge */ + withName: '.*SORTBAM' { + ext.args = '--sort-order TemplateCoordinate' + ext.prefix = { "${meta.id}.sorted" } + publishDir = [ + path: { "${params.outdir}/preprocessing/merged/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: '*.bam' + ] + } + withName: '.*GROUPREADSBYUMI' { publishDir = [ [ diff --git a/modules.json b/modules.json index 521214e..277e8ec 100644 --- a/modules.json +++ b/modules.json @@ -15,6 +15,11 @@ "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", "installed_by": ["modules"] }, + "fgbio/sortbam": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", diff --git a/modules/nf-core/fgbio/sortbam/environment.yml b/modules/nf-core/fgbio/sortbam/environment.yml new file mode 100644 index 0000000..ddc8a41 --- /dev/null +++ b/modules/nf-core/fgbio/sortbam/environment.yml @@ -0,0 +1,7 @@ +name: fgbio_sortbam +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fgbio=2.0.2 diff --git a/modules/nf-core/fgbio/sortbam/main.nf b/modules/nf-core/fgbio/sortbam/main.nf new file mode 100644 index 0000000..3c779d0 --- /dev/null +++ b/modules/nf-core/fgbio/sortbam/main.nf @@ -0,0 +1,36 @@ +process FGBIO_SORTBAM { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/fgbio:2.0.2--hdfd78af_0' : + 'biocontainers/fgbio:2.0.2--hdfd78af_0' }" + + input: + tuple val(meta), path(bam) + + output: + tuple val(meta), path("*.bam"), emit: bam + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + fgbio \\ + --tmp-dir=. \\ + SortBam \\ + -i $bam \\ + $args \\ + -o ${prefix}.bam + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') + END_VERSIONS + """ +} + diff --git a/modules/nf-core/fgbio/sortbam/meta.yml b/modules/nf-core/fgbio/sortbam/meta.yml new file mode 100644 index 0000000..61630d7 --- /dev/null +++ b/modules/nf-core/fgbio/sortbam/meta.yml @@ -0,0 +1,42 @@ +name: fgbio_sortbam +description: Sorts a SAM or BAM file. Several sort orders are available, including coordinate, queryname, random, and randomquery. +keywords: + - sort + - bam + - sam +tools: + - fgbio: + description: Tools for working with genomic and high throughput sequencing data. + homepage: https://github.com/fulcrumgenomics/fgbio + documentation: http://fulcrumgenomics.github.io/fgbio/ + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false, collapse:false ] + - bam: + type: file + description: | + The input SAM or BAM file to be sorted. + pattern: "*.{bam,sam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: | + Output SAM or BAM file. + pattern: "*.{bam,sam}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@sruthipsuresh" +maintainers: + - "@sruthipsuresh" diff --git a/tests/pipeline/multi_lanes.nf.test b/tests/pipeline/multi_lanes.nf.test index f8105c1..64e2e83 100644 --- a/tests/pipeline/multi_lanes.nf.test +++ b/tests/pipeline/multi_lanes.nf.test @@ -20,7 +20,7 @@ nextflow_pipeline { assertAll( { assert workflow.success }, { assert snapshot(UTILS.removeNextflowVersion("$outputDir")).match("multi_lanes_rd_software_versions") }, - { assert workflow.trace.succeeded().size() == 39 }, + { assert workflow.trace.succeeded().size() == 41 }, { assert new File("$outputDir/multiqc/multiqc_report.html").exists() }, { assert new File("$outputDir/consensus_filtering/filtered/SRR6109255_one_lane/SRR6109255_one_lane.cons.filtered.bam").exists() }, { assert new File("$outputDir/consensus_filtering/filtered/SRR6109255_two_lanes/SRR6109255_two_lanes.cons.filtered.bam").exists() }, @@ -53,7 +53,7 @@ nextflow_pipeline { assertAll( { assert workflow.success }, { assert snapshot(UTILS.removeNextflowVersion("$outputDir")).match("multi_lanes_ht_software_versions") }, - { assert workflow.trace.succeeded().size() == 36 }, + { assert workflow.trace.succeeded().size() == 38 }, { assert new File("$outputDir/multiqc/multiqc_report.html").exists() }, { assert new File("$outputDir/consensus_filtering/filtered/SRR6109255_one_lane/SRR6109255_one_lane.cons.filtered.bam").exists() }, { assert new File("$outputDir/consensus_filtering/filtered/SRR6109255_two_lanes/SRR6109255_two_lanes.cons.filtered.bam").exists() }, diff --git a/tests/pipeline/multi_lanes.nf.test.snap b/tests/pipeline/multi_lanes.nf.test.snap index d48b1b3..399dd44 100644 --- a/tests/pipeline/multi_lanes.nf.test.snap +++ b/tests/pipeline/multi_lanes.nf.test.snap @@ -1,13 +1,13 @@ { "multi_lanes_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, SORTBAM={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.0dev}}" ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-05-20T15:50:47.281159" + "timestamp": "2024-05-30T00:51:13.471176" }, "multi_lanes_ht": { "content": [ @@ -26,13 +26,13 @@ }, "multi_lanes_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, SORTBAM={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.0dev}}" ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-05-20T15:58:15.728901" + "timestamp": "2024-05-30T00:59:50.302089" }, "multi_lanes_rd": { "content": [ diff --git a/workflows/fastquorum.nf b/workflows/fastquorum.nf index 1f2da64..5f69526 100644 --- a/workflows/fastquorum.nf +++ b/workflows/fastquorum.nf @@ -21,6 +21,7 @@ include { FGBIO_COLLECTDUPLEXSEQMETRICS as COLLECTDUPLEXSEQMETRICS include { FGBIO_CALLANDFILTERMOLECULARCONSENSUSREADS as CALLANDFILTERMOLECULARCONSENSUSREADS } from '../modules/local/fgbio/callandfiltermolecularconsensusreads/main' include { FGBIO_CALLANDFILTERDUPLEXCONSENSUSREADS as CALLANDFILTERDUPLEXCONSENSUSREADS } from '../modules/local/fgbio/callandfilterduplexconsensusreads/main' include { SAMTOOLS_MERGE as MERGE_BAM } from '../modules/nf-core/samtools/merge/main' +include { FGBIO_SORTBAM as SORTBAM } from '../modules/nf-core/fgbio/sortbam/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' @@ -92,10 +93,17 @@ workflow FASTQUORUM { MERGE_BAM(bam_to_merge.multiple, [[], []], [[], []]) ch_versions = ch_versions.mix(MERGE_BAM.out.versions.first()) + // + // MODULE: Run fgbio SortBam to re-sort into TemplateCoordinate. This can be removed when samtools is released + // with the following bugfix: https://github.com/samtools/samtools/pull/2062 + // + SORTBAM(MERGE_BAM.out.bam) + ch_versions = ch_versions.mix(SORTBAM.out.versions.first()) + // // Create a channel that contains the merged BAMs and those that did not need to be merged. // - bam_all = MERGE_BAM.out.bam.mix(bam_to_merge.single) + bam_all = SORTBAM.out.bam.mix(bam_to_merge.single) // // MODULE: Run fgbio GroupReadsByUmi From 223c545c9341840612c98cfb7b27e2d84d888eca Mon Sep 17 00:00:00 2001 From: Nils Homer Date: Thu, 1 Aug 2024 19:51:31 -0700 Subject: [PATCH 18/30] Apply suggestions from code review Co-authored-by: Simon Pearce <24893913+SPPearce@users.noreply.github.com> --- conf/modules.config | 2 +- tests/pipeline/multi_lanes.nf.test.snap | 4 ++-- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 6d908fb..68b7b8d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -88,7 +88,7 @@ process { ] } - /* This can be removed when https://github.com/samtools/samtools/pull/2062 is merge */ + /* This can be removed when https://github.com/samtools/samtools/pull/2062 is merged */ withName: '.*SORTBAM' { ext.args = '--sort-order TemplateCoordinate' ext.prefix = { "${meta.id}.sorted" } diff --git a/tests/pipeline/multi_lanes.nf.test.snap b/tests/pipeline/multi_lanes.nf.test.snap index 399dd44..658e3d5 100644 --- a/tests/pipeline/multi_lanes.nf.test.snap +++ b/tests/pipeline/multi_lanes.nf.test.snap @@ -1,7 +1,7 @@ { "multi_lanes_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, SORTBAM={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.0dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, SORTBAM={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", @@ -26,7 +26,7 @@ }, "multi_lanes_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, SORTBAM={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.0dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, SORTBAM={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" ], "meta": { "nf-test": "0.8.4", From 46ff48d9a97d82733f85323c27314623c475fc0d Mon Sep 17 00:00:00 2001 From: znorgaard Date: Thu, 22 Aug 2024 14:50:18 -0700 Subject: [PATCH 19/30] chore: update module versions --- docs/output.md | 2 - modules.json | 14 +- modules/nf-core/bwa/index/meta.yml | 2 +- modules/nf-core/fastqc/main.nf | 5 +- modules/nf-core/fastqc/tests/main.nf.test | 225 ++++++++--- .../nf-core/fastqc/tests/main.nf.test.snap | 370 ++++++++++++++++-- modules/nf-core/fgbio/sortbam/environment.yml | 2 +- modules/nf-core/fgbio/sortbam/main.nf | 35 +- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 12 +- modules/nf-core/multiqc/meta.yml | 13 + modules/nf-core/multiqc/tests/main.nf.test | 8 + .../nf-core/multiqc/tests/main.nf.test.snap | 20 +- modules/nf-core/samtools/dict/environment.yml | 4 +- modules/nf-core/samtools/dict/main.nf | 4 +- .../nf-core/samtools/faidx/environment.yml | 4 +- modules/nf-core/samtools/faidx/main.nf | 4 +- .../samtools/faidx/tests/main.nf.test.snap | 30 +- .../nf-core/samtools/merge/environment.yml | 4 +- modules/nf-core/samtools/merge/main.nf | 4 +- .../samtools/merge/tests/main.nf.test.snap | 24 +- 21 files changed, 622 insertions(+), 166 deletions(-) diff --git a/docs/output.md b/docs/output.md index d596f9d..a519d84 100644 --- a/docs/output.md +++ b/docs/output.md @@ -6,8 +6,6 @@ This document describes the output produced by the pipeline. Most of the plots a The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. - - ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: diff --git a/modules.json b/modules.json index 277e8ec..7469921 100644 --- a/modules.json +++ b/modules.json @@ -7,37 +7,37 @@ "nf-core": { "bwa/index": { "branch": "master", - "git_sha": "086fa66260595e123b0ea47a6512539b72a9afa3", + "git_sha": "e0ff65e1fb313677de09f5f477ae3da30ce19b7b", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", + "git_sha": "b49b8992e5271ce427f3a7cdb29628fc58400eb5", "installed_by": ["modules"] }, "fgbio/sortbam": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "2fc7438d5dd0a4305fd68afcb8a36acd70234614", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", + "git_sha": "fe9614c5d1d9820dae56a5e30a07ba336db45835", "installed_by": ["modules"] }, "samtools/dict": { "branch": "master", - "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", + "git_sha": "3c8fd07ea8d86daaed24fddfa76cd8ba2cd37c9b", "installed_by": ["modules"] }, "samtools/faidx": { "branch": "master", - "git_sha": "f153f1f10e1083c49935565844cccb7453021682", + "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", "installed_by": ["modules"] }, "samtools/merge": { "branch": "master", - "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", + "git_sha": "04fbbc7c43cebc0b95d5b126f6d9fe4effa33519", "installed_by": ["modules"] } } diff --git a/modules/nf-core/bwa/index/meta.yml b/modules/nf-core/bwa/index/meta.yml index 4c7d30f..6bbc87a 100644 --- a/modules/nf-core/bwa/index/meta.yml +++ b/modules/nf-core/bwa/index/meta.yml @@ -11,7 +11,7 @@ tools: BWA is a software package for mapping DNA sequences against a large reference genome, such as the human genome. homepage: http://bio-bwa.sourceforge.net/ - documentation: http://www.htslib.org/doc/samtools.html + documentation: https://bio-bwa.sourceforge.net/bwa.shtml arxiv: arXiv:1303.3997 licence: ["GPL-3.0-or-later"] input: diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index d79f1c8..d8989f4 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -26,7 +26,10 @@ process FASTQC { def rename_to = old_new_pairs*.join(' ').join(' ') def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') - def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') + // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory) + // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222 + // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label + def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') / task.cpus // FastQC memory value allowed range (100 - 10000) def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb) diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 70edae4..e9d79a0 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -23,17 +23,14 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. - // looks like this:

- // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_single") } + { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:
Mon 2 Oct 2023
test.gz
+ // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -54,16 +51,14 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_paired") } + { assert process.success }, + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -83,13 +78,11 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } + { assert process.success }, + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -109,13 +102,11 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_bam") } + { assert process.success }, + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -138,22 +129,20 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, - { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, - { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][2]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][3]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } + { assert process.success }, + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][2]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][3]).text.contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -173,21 +162,18 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } + { assert process.success }, + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match() } ) } } test("sarscov2 single-end [fastq] - stub") { - options "-stub" - + options "-stub" when { process { """ @@ -201,12 +187,123 @@ nextflow_process { then { assertAll ( - { assert process.success }, - { assert snapshot(process.out.html.collect { file(it[1]).getName() } + - process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match("fastqc_stub") } + { assert process.success }, + { assert snapshot(process.out).match() } ) } } + test("sarscov2 paired-end [fastq] - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("sarscov2 interleaved [fastq] - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("sarscov2 paired-end [bam] - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("sarscov2 multiple [fastq] - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("sarscov2 custom_prefix - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 86f7c31..d5db309 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,88 +1,392 @@ { - "fastqc_versions_interleaved": { + "sarscov2 custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:40:07.293713" + "timestamp": "2024-07-22T11:02:16.374038" }, - "fastqc_stub": { + "sarscov2 single-end [fastq] - stub": { "content": [ - [ - "test.html", - "test.zip", - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": true + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": true + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": true + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:02:24.993809" + }, + "sarscov2 custom_prefix - stub": { + "content": [ + { + "0": [ + [ + { + "id": "mysample", + "single_end": true + }, + "mysample.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "mysample", + "single_end": true + }, + "mysample.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "mysample", + "single_end": true + }, + "mysample.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "mysample", + "single_end": true + }, + "mysample.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:31:01.425198" + "timestamp": "2024-07-22T11:03:10.93942" }, - "fastqc_versions_multiple": { + "sarscov2 interleaved [fastq]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:40:55.797907" + "timestamp": "2024-07-22T11:01:42.355718" }, - "fastqc_versions_bam": { + "sarscov2 paired-end [bam]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:40:26.795862" + "timestamp": "2024-07-22T11:01:53.276274" }, - "fastqc_versions_single": { + "sarscov2 multiple [fastq]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:39:27.043675" + "timestamp": "2024-07-22T11:02:05.527626" }, - "fastqc_versions_paired": { + "sarscov2 paired-end [fastq]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:01:31.188871" + }, + "sarscov2 paired-end [fastq] - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:02:34.273566" + }, + "sarscov2 multiple [fastq] - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:39:47.584191" + "timestamp": "2024-07-22T11:03:02.304411" }, - "fastqc_versions_custom_prefix": { + "sarscov2 single-end [fastq]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:01:19.095607" + }, + "sarscov2 interleaved [fastq] - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:02:44.640184" + }, + "sarscov2 paired-end [bam] - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:41:14.576531" + "timestamp": "2024-07-22T11:02:53.550742" } } \ No newline at end of file diff --git a/modules/nf-core/fgbio/sortbam/environment.yml b/modules/nf-core/fgbio/sortbam/environment.yml index ddc8a41..452613a 100644 --- a/modules/nf-core/fgbio/sortbam/environment.yml +++ b/modules/nf-core/fgbio/sortbam/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::fgbio=2.0.2 + - bioconda::fgbio=2.2.1 diff --git a/modules/nf-core/fgbio/sortbam/main.nf b/modules/nf-core/fgbio/sortbam/main.nf index 3c779d0..e061f7c 100644 --- a/modules/nf-core/fgbio/sortbam/main.nf +++ b/modules/nf-core/fgbio/sortbam/main.nf @@ -4,8 +4,8 @@ process FGBIO_SORTBAM { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fgbio:2.0.2--hdfd78af_0' : - 'biocontainers/fgbio:2.0.2--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/fgbio:2.2.1--hdfd78af_0' : + 'biocontainers/fgbio:2.2.1--hdfd78af_0' }" input: tuple val(meta), path(bam) @@ -19,9 +19,23 @@ process FGBIO_SORTBAM { script: def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" + def prefix = task.ext.prefix ?: "${meta.id}_sorted" + def mem_gb = 8 + if (!task.memory) { + log.info '[fgbio SortBam] Available memory not known - defaulting to 8GB. Specify process memory requirements to change this.' + } else if (mem_gb > task.memory.giga) { + if (task.memory.giga < 2) { + mem_gb = 1 + } else { + mem_gb = task.memory.giga - 1 + } + } + + if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ - fgbio \\ + fgbio -Xmx${mem_gb}g \\ + --async-io=true \\ --tmp-dir=. \\ SortBam \\ -i $bam \\ @@ -32,5 +46,16 @@ process FGBIO_SORTBAM { fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') END_VERSIONS """ -} + stub: + prefix = task.ext.prefix ?: "${meta.id}_sorted" + if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + touch ${prefix}.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index ca39fb6..a31464c 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.21 + - bioconda::multiqc=1.24.1 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 47ac352..d536580 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,14 +3,16 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : - 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.24.1--pyhdfd78af_0' : + 'biocontainers/multiqc:1.24.1--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" path(multiqc_config) path(extra_multiqc_config) path(multiqc_logo) + path(replace_names) + path(sample_names) output: path "*multiqc_report.html", emit: report @@ -23,16 +25,22 @@ process MULTIQC { script: def args = task.ext.args ?: '' + def prefix = task.ext.prefix ? "--filename ${task.ext.prefix}.html" : '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' + def replace = replace_names ? "--replace-names ${replace_names}" : '' + def samples = sample_names ? "--sample-names ${sample_names}" : '' """ multiqc \\ --force \\ $args \\ $config \\ + $prefix \\ $extra_config \\ $logo \\ + $replace \\ + $samples \\ . cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index 45a9bc3..382c08c 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -29,6 +29,19 @@ input: type: file description: Optional logo file for MultiQC pattern: "*.{png}" + - replace_names: + type: file + description: | + Optional two-column sample renaming file. First column a set of + patterns, second column a set of corresponding replacements. Passed via + MultiQC's `--replace-names` option. + pattern: "*.{tsv}" + - sample_names: + type: file + description: | + Optional TSV file with headers, passed to the MultiQC --sample_names + argument. + pattern: "*.{tsv}" output: - report: type: file diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index f1c4242..33316a7 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -8,6 +8,8 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" + config "./nextflow.config" + test("sarscov2 single-end [fastqc]") { when { @@ -17,6 +19,8 @@ nextflow_process { input[1] = [] input[2] = [] input[3] = [] + input[4] = [] + input[5] = [] """ } } @@ -41,6 +45,8 @@ nextflow_process { input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] + input[4] = [] + input[5] = [] """ } } @@ -66,6 +72,8 @@ nextflow_process { input[1] = [] input[2] = [] input[3] = [] + input[4] = [] + input[5] = [] """ } } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index bfebd80..83fa080 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.2" }, - "timestamp": "2024-02-29T08:48:55.657331" + "timestamp": "2024-07-10T12:41:34.562023" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.2" }, - "timestamp": "2024-02-29T08:49:49.071937" + "timestamp": "2024-07-10T11:27:11.933869532" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.2" }, - "timestamp": "2024-02-29T08:49:25.457567" + "timestamp": "2024-07-10T11:26:56.709849369" } -} \ No newline at end of file +} diff --git a/modules/nf-core/samtools/dict/environment.yml b/modules/nf-core/samtools/dict/environment.yml index aca9748..14a893c 100644 --- a/modules/nf-core/samtools/dict/environment.yml +++ b/modules/nf-core/samtools/dict/environment.yml @@ -4,5 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::samtools=1.19.2 - - bioconda::htslib=1.19.1 + - bioconda::samtools=1.20 + - bioconda::htslib=1.20 diff --git a/modules/nf-core/samtools/dict/main.nf b/modules/nf-core/samtools/dict/main.nf index 13dd6bf..52be415 100644 --- a/modules/nf-core/samtools/dict/main.nf +++ b/modules/nf-core/samtools/dict/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_DICT { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : - 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : + 'biocontainers/samtools:1.20--h50ea8bc_0' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/samtools/faidx/environment.yml b/modules/nf-core/samtools/faidx/environment.yml index 9c24eb0..f8450fa 100644 --- a/modules/nf-core/samtools/faidx/environment.yml +++ b/modules/nf-core/samtools/faidx/environment.yml @@ -6,5 +6,5 @@ channels: - defaults dependencies: - - bioconda::htslib=1.19.1 - - bioconda::samtools=1.19.2 + - bioconda::htslib=1.20 + - bioconda::samtools=1.20 diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf index cfe7ad9..bdcdbc9 100644 --- a/modules/nf-core/samtools/faidx/main.nf +++ b/modules/nf-core/samtools/faidx/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_FAIDX { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : - 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : + 'biocontainers/samtools:1.20--h50ea8bc_0' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/samtools/faidx/tests/main.nf.test.snap b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap index 3e651ef..3223b72 100644 --- a/modules/nf-core/samtools/faidx/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/faidx/tests/main.nf.test.snap @@ -18,7 +18,7 @@ ], "3": [ - "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c" + "versions.yml:md5,2db78952923a61e05d50b95518b21856" ], "fa": [ @@ -36,7 +36,7 @@ ], "versions": [ - "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c" + "versions.yml:md5,2db78952923a61e05d50b95518b21856" ] } ], @@ -44,7 +44,7 @@ "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-03-18T16:22:39.412601" + "timestamp": "2024-05-28T15:42:14.779784761" }, "test_samtools_faidx_bgzip": { "content": [ @@ -71,7 +71,7 @@ ] ], "3": [ - "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c" + "versions.yml:md5,2db78952923a61e05d50b95518b21856" ], "fa": [ @@ -95,7 +95,7 @@ ] ], "versions": [ - "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c" + "versions.yml:md5,2db78952923a61e05d50b95518b21856" ] } ], @@ -103,7 +103,7 @@ "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-03-18T16:23:22.427966" + "timestamp": "2024-05-28T15:42:20.256633877" }, "test_samtools_faidx_fasta": { "content": [ @@ -124,7 +124,7 @@ ], "3": [ - "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c" + "versions.yml:md5,2db78952923a61e05d50b95518b21856" ], "fa": [ [ @@ -142,7 +142,7 @@ ], "versions": [ - "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c" + "versions.yml:md5,2db78952923a61e05d50b95518b21856" ] } ], @@ -150,7 +150,7 @@ "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-03-18T16:24:04.107537" + "timestamp": "2024-05-28T15:42:25.632577273" }, "test_samtools_faidx_stub_fasta": { "content": [ @@ -171,7 +171,7 @@ ], "3": [ - "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c" + "versions.yml:md5,2db78952923a61e05d50b95518b21856" ], "fa": [ [ @@ -189,7 +189,7 @@ ], "versions": [ - "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c" + "versions.yml:md5,2db78952923a61e05d50b95518b21856" ] } ], @@ -197,7 +197,7 @@ "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-03-18T16:24:45.868463" + "timestamp": "2024-05-28T15:42:31.058424849" }, "test_samtools_faidx_stub_fai": { "content": [ @@ -218,7 +218,7 @@ ], "3": [ - "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c" + "versions.yml:md5,2db78952923a61e05d50b95518b21856" ], "fa": [ @@ -236,7 +236,7 @@ ], "versions": [ - "versions.yml:md5,4870fc0a88c616aa937f8325a2db0c3c" + "versions.yml:md5,2db78952923a61e05d50b95518b21856" ] } ], @@ -244,6 +244,6 @@ "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-03-18T16:25:27.550554" + "timestamp": "2024-05-28T15:42:36.479929617" } } \ No newline at end of file diff --git a/modules/nf-core/samtools/merge/environment.yml b/modules/nf-core/samtools/merge/environment.yml index fc669b1..cd366d6 100644 --- a/modules/nf-core/samtools/merge/environment.yml +++ b/modules/nf-core/samtools/merge/environment.yml @@ -4,5 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::samtools=1.19.2 - - bioconda::htslib=1.19.1 + - bioconda::samtools=1.20 + - bioconda::htslib=1.20 diff --git a/modules/nf-core/samtools/merge/main.nf b/modules/nf-core/samtools/merge/main.nf index a3048c2..693b1d8 100644 --- a/modules/nf-core/samtools/merge/main.nf +++ b/modules/nf-core/samtools/merge/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_MERGE { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : - 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.20--h50ea8bc_0' : + 'biocontainers/samtools:1.20--h50ea8bc_0' }" input: tuple val(meta), path(input_files, stageAs: "?/*") diff --git a/modules/nf-core/samtools/merge/tests/main.nf.test.snap b/modules/nf-core/samtools/merge/tests/main.nf.test.snap index f7da769..17bc846 100644 --- a/modules/nf-core/samtools/merge/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/merge/tests/main.nf.test.snap @@ -80,14 +80,14 @@ "bam_versions": { "content": [ [ - "versions.yml:md5,52c62d4712f7af00eb962d090ca32fe4" + "versions.yml:md5,84dab54b9812780df48f5cecef690c34" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "24.01.0" + "nextflow": "23.10.1" }, - "timestamp": "2024-02-13T16:16:33.782637377" + "timestamp": "2024-05-28T15:46:35.851936597" }, "bams_csi": { "content": [ @@ -124,14 +124,14 @@ "bams_stub_versions": { "content": [ [ - "versions.yml:md5,52c62d4712f7af00eb962d090ca32fe4" + "versions.yml:md5,84dab54b9812780df48f5cecef690c34" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "24.01.0" + "nextflow": "23.10.1" }, - "timestamp": "2024-02-13T16:16:42.594476052" + "timestamp": "2024-05-28T15:46:41.405707643" }, "bam_cram": { "content": [ @@ -158,14 +158,14 @@ "bams_versions": { "content": [ [ - "versions.yml:md5,52c62d4712f7af00eb962d090ca32fe4" + "versions.yml:md5,84dab54b9812780df48f5cecef690c34" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "24.01.0" + "nextflow": "23.10.1" }, - "timestamp": "2024-02-13T16:16:04.805335656" + "timestamp": "2024-05-28T15:45:51.695689923" }, "crams_bam": { "content": [ @@ -182,14 +182,14 @@ "crams_versions": { "content": [ [ - "versions.yml:md5,52c62d4712f7af00eb962d090ca32fe4" + "versions.yml:md5,84dab54b9812780df48f5cecef690c34" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "24.01.0" + "nextflow": "23.10.1" }, - "timestamp": "2024-02-13T16:16:25.889394689" + "timestamp": "2024-05-28T15:46:30.185392319" }, "bam_csi": { "content": [ From 7ae7a6e1572f6d61276a9c5483477829a0afdbe2 Mon Sep 17 00:00:00 2001 From: znorgaard Date: Thu, 22 Aug 2024 15:06:18 -0700 Subject: [PATCH 20/30] chore: update changelog --- CHANGELOG.md | 9 +++------ 1 file changed, 3 insertions(+), 6 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 69fda89..31a6609 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## Unreleased +## [[1.0.1]](https://github.com/nf-core/fastquorum/releases/tag/1.0.1) -- 2024-08-?? ### Enhancements & fixes @@ -11,12 +11,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [PR #58](https://github.com/nf-core/fastquorum/pull/58) - Prepare genome steps now run only if the corresponding parameters are not passed. - [PR #60](https://github.com/nf-core/fastquorum/pull/60) - Enable automatic escalation of memory for FilterConsensusReads. - [PR #67](https://github.com/nf-core/fastquorum/pull/67) - Fix setting the parameters from igenomes. +- [PR #68](https://github.com/nf-core/fastquorum/pull/68) - Temporary fix to merging BAMs across lanes in template-coordinate order. Using `fgbio SortBam` after `samtools merge`. Related to [samtools#2062](https://github.com/samtools/samtools/pull/2062). -### Enhancements & fixes - -- [PR #54](https://github.com/nf-core/fastquorum/pull/54) - Temporary fix to merging BAMs across lanes in template-coordinate order. Using `fgbio SortBam` after `samtools merge`. Related to [samtools#2062](https://github.com/samtools/samtools/pull/2062). - -## [[1.0.0]](https://github.com/nf-core/fastquorum/releases/tag/1.0.0)] -- 2024-05-20 +## [[1.0.0]](https://github.com/nf-core/fastquorum/releases/tag/1.0.0) -- 2024-05-20 Initial release of nf-core/fastquorum, created with the [nf-core](https://nf-co.re/) template. From a154baa031b1470b100aed086aae523e2681e0cc Mon Sep 17 00:00:00 2001 From: znorgaard Date: Thu, 22 Aug 2024 15:11:06 -0700 Subject: [PATCH 21/30] chore: update version --- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- tests/pipeline/multi_fastq.nf.test.snap | 4 ++-- tests/pipeline/multi_lanes.nf.test.snap | 4 ++-- tests/pipeline/single_fastq.nf.test.snap | 4 ++-- tests/pipeline/tiny.nf.test.snap | 4 ++-- 6 files changed, 11 insertions(+), 11 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index c2b23db..dcb8b27 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/fastquorum + This report has been generated by the nf-core/fastquorum analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-fastquorum-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index c7cc4fb..ef0d178 100644 --- a/nextflow.config +++ b/nextflow.config @@ -250,7 +250,7 @@ manifest { description = """fgbio Best Practices FASTQ to Consensus Pipeline""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.0.1dev' + version = '1.0.1' doi = '10.5281/zenodo.10456900,10.5281/zenodo.11267672' } diff --git a/tests/pipeline/multi_fastq.nf.test.snap b/tests/pipeline/multi_fastq.nf.test.snap index 06c4533..73b9ee0 100644 --- a/tests/pipeline/multi_fastq.nf.test.snap +++ b/tests/pipeline/multi_fastq.nf.test.snap @@ -16,7 +16,7 @@ }, "multi_fastq_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" ], "meta": { "nf-test": "0.8.4", @@ -26,7 +26,7 @@ }, "multi_fastq_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" ], "meta": { "nf-test": "0.8.4", diff --git a/tests/pipeline/multi_lanes.nf.test.snap b/tests/pipeline/multi_lanes.nf.test.snap index 658e3d5..156c3cd 100644 --- a/tests/pipeline/multi_lanes.nf.test.snap +++ b/tests/pipeline/multi_lanes.nf.test.snap @@ -1,7 +1,7 @@ { "multi_lanes_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, SORTBAM={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, SORTBAM={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" ], "meta": { "nf-test": "0.8.4", @@ -26,7 +26,7 @@ }, "multi_lanes_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, SORTBAM={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, SORTBAM={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" ], "meta": { "nf-test": "0.8.4", diff --git a/tests/pipeline/single_fastq.nf.test.snap b/tests/pipeline/single_fastq.nf.test.snap index 9a82518..2b1e42f 100644 --- a/tests/pipeline/single_fastq.nf.test.snap +++ b/tests/pipeline/single_fastq.nf.test.snap @@ -11,7 +11,7 @@ }, "single_fastq_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" ], "meta": { "nf-test": "0.8.4", @@ -21,7 +21,7 @@ }, "single_fastq_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" ], "meta": { "nf-test": "0.8.4", diff --git a/tests/pipeline/tiny.nf.test.snap b/tests/pipeline/tiny.nf.test.snap index 32dcb04..7718da2 100644 --- a/tests/pipeline/tiny.nf.test.snap +++ b/tests/pipeline/tiny.nf.test.snap @@ -1,7 +1,7 @@ { "tiny_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" ], "meta": { "nf-test": "0.8.4", @@ -11,7 +11,7 @@ }, "tiny_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1dev}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" ], "meta": { "nf-test": "0.8.4", From 6f75578274de53100b7da4f9bd9a1d4b4619b1bc Mon Sep 17 00:00:00 2001 From: znorgaard Date: Thu, 22 Aug 2024 15:19:55 -0700 Subject: [PATCH 22/30] chore: add contributors --- CHANGELOG.md | 8 ++++++++ 1 file changed, 8 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 31a6609..1580d5f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -5,6 +5,14 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## [[1.0.1]](https://github.com/nf-core/fastquorum/releases/tag/1.0.1) -- 2024-08-?? +### Credits + +Special thanks to the following for their contributions to the release: + +- [Nils Homer](https://github.com/nh13) +- [Simon Pearce](https://github.com/SPPearce) +- [Zach Norgaard](https://github.com/znorgaard) + ### Enhancements & fixes - [PR #51](https://github.com/nf-core/fastquorum/pull/51) - Fixes a bug where alignment and filtering where swapped in the phase 2 high-throughput diagrams (@jfy133). From cc8c00e1d5825e9eb9ba8a4416d9a93cfae75d7d Mon Sep 17 00:00:00 2001 From: znorgaard Date: Fri, 23 Aug 2024 08:10:05 -0700 Subject: [PATCH 23/30] fix: downgrade multiqc --- modules.json | 2 +- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 12 ++--------- modules/nf-core/multiqc/meta.yml | 13 ------------ modules/nf-core/multiqc/tests/main.nf.test | 8 -------- .../nf-core/multiqc/tests/main.nf.test.snap | 20 +++++++++---------- 6 files changed, 14 insertions(+), 43 deletions(-) diff --git a/modules.json b/modules.json index 7469921..4773f1f 100644 --- a/modules.json +++ b/modules.json @@ -22,7 +22,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "fe9614c5d1d9820dae56a5e30a07ba336db45835", + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", "installed_by": ["modules"] }, "samtools/dict": { diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index a31464c..ca39fb6 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.24.1 + - bioconda::multiqc=1.21 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index d536580..47ac352 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,16 +3,14 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.24.1--pyhdfd78af_0' : - 'biocontainers/multiqc:1.24.1--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : + 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" path(multiqc_config) path(extra_multiqc_config) path(multiqc_logo) - path(replace_names) - path(sample_names) output: path "*multiqc_report.html", emit: report @@ -25,22 +23,16 @@ process MULTIQC { script: def args = task.ext.args ?: '' - def prefix = task.ext.prefix ? "--filename ${task.ext.prefix}.html" : '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' - def replace = replace_names ? "--replace-names ${replace_names}" : '' - def samples = sample_names ? "--sample-names ${sample_names}" : '' """ multiqc \\ --force \\ $args \\ $config \\ - $prefix \\ $extra_config \\ $logo \\ - $replace \\ - $samples \\ . cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index 382c08c..45a9bc3 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -29,19 +29,6 @@ input: type: file description: Optional logo file for MultiQC pattern: "*.{png}" - - replace_names: - type: file - description: | - Optional two-column sample renaming file. First column a set of - patterns, second column a set of corresponding replacements. Passed via - MultiQC's `--replace-names` option. - pattern: "*.{tsv}" - - sample_names: - type: file - description: | - Optional TSV file with headers, passed to the MultiQC --sample_names - argument. - pattern: "*.{tsv}" output: - report: type: file diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index 33316a7..f1c4242 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -8,8 +8,6 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" - config "./nextflow.config" - test("sarscov2 single-end [fastqc]") { when { @@ -19,8 +17,6 @@ nextflow_process { input[1] = [] input[2] = [] input[3] = [] - input[4] = [] - input[5] = [] """ } } @@ -45,8 +41,6 @@ nextflow_process { input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] - input[4] = [] - input[5] = [] """ } } @@ -72,8 +66,6 @@ nextflow_process { input[1] = [] input[2] = [] input[3] = [] - input[4] = [] - input[5] = [] """ } } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index 83fa080..bfebd80 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nextflow": "23.10.1" }, - "timestamp": "2024-07-10T12:41:34.562023" + "timestamp": "2024-02-29T08:48:55.657331" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nextflow": "23.10.1" }, - "timestamp": "2024-07-10T11:27:11.933869532" + "timestamp": "2024-02-29T08:49:49.071937" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nextflow": "23.10.1" }, - "timestamp": "2024-07-10T11:26:56.709849369" + "timestamp": "2024-02-29T08:49:25.457567" } -} +} \ No newline at end of file From eb51ca62cfb08636e29d422a2e2777cce0badffe Mon Sep 17 00:00:00 2001 From: znorgaard Date: Fri, 23 Aug 2024 09:29:32 -0700 Subject: [PATCH 24/30] chore: update multiqc module --- modules.json | 4 +- .../nf-core/fgbio/sortbam/tests/main.nf.test | 56 +++++++++++++++ .../fgbio/sortbam/tests/main.nf.test.snap | 68 +++++++++++++++++++ modules/nf-core/fgbio/sortbam/tests/tags.yml | 2 + modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 12 +++- modules/nf-core/multiqc/meta.yml | 13 ++++ modules/nf-core/multiqc/tests/main.nf.test | 8 +++ .../nf-core/multiqc/tests/main.nf.test.snap | 20 +++--- modules/nf-core/multiqc/tests/nextflow.config | 5 ++ .../nf-core/samtools/dict/tests/main.nf.test | 62 +++++++++++++++++ .../samtools/dict/tests/main.nf.test.snap | 50 ++++++++++++++ nextflow.config | 10 +-- nextflow_schema.json | 14 ++++ workflows/fastquorum.nf | 7 +- 15 files changed, 313 insertions(+), 20 deletions(-) create mode 100644 modules/nf-core/fgbio/sortbam/tests/main.nf.test create mode 100644 modules/nf-core/fgbio/sortbam/tests/main.nf.test.snap create mode 100644 modules/nf-core/fgbio/sortbam/tests/tags.yml create mode 100644 modules/nf-core/multiqc/tests/nextflow.config create mode 100644 modules/nf-core/samtools/dict/tests/main.nf.test create mode 100644 modules/nf-core/samtools/dict/tests/main.nf.test.snap diff --git a/modules.json b/modules.json index 4773f1f..7851eca 100644 --- a/modules.json +++ b/modules.json @@ -22,7 +22,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", + "git_sha": "fe9614c5d1d9820dae56a5e30a07ba336db45835", "installed_by": ["modules"] }, "samtools/dict": { @@ -46,7 +46,7 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "git_sha": "20c03aede5a80ff520a905cea1f8ca121b5bb661", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { diff --git a/modules/nf-core/fgbio/sortbam/tests/main.nf.test b/modules/nf-core/fgbio/sortbam/tests/main.nf.test new file mode 100644 index 0000000..2e9b245 --- /dev/null +++ b/modules/nf-core/fgbio/sortbam/tests/main.nf.test @@ -0,0 +1,56 @@ +nextflow_process { + + name "Test Process FGBIO_SORTBAM" + script "../main.nf" + process "FGBIO_SORTBAM" + + tag "modules" + tag "modules_nfcore" + tag "fgbio" + tag "fgbio/sortbam" + + test("sarscov2 - bam") { + + when { + process { + """ + input[0] = [ [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - bam - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/fgbio/sortbam/tests/main.nf.test.snap b/modules/nf-core/fgbio/sortbam/tests/main.nf.test.snap new file mode 100644 index 0000000..39761a9 --- /dev/null +++ b/modules/nf-core/fgbio/sortbam/tests/main.nf.test.snap @@ -0,0 +1,68 @@ +{ + "sarscov2 - bam - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test_sorted.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,384c5398cae017e4fe0df8cb8f004017" + ], + "bam": [ + [ + { + "id": "test" + }, + "test_sorted.bam:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,384c5398cae017e4fe0df8cb8f004017" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-21T05:37:31.605753331" + }, + "sarscov2 - bam": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test_sorted.bam:md5,1d7a558a72b7aecc80946cb9cadf8f60" + ] + ], + "1": [ + "versions.yml:md5,384c5398cae017e4fe0df8cb8f004017" + ], + "bam": [ + [ + { + "id": "test" + }, + "test_sorted.bam:md5,1d7a558a72b7aecc80946cb9cadf8f60" + ] + ], + "versions": [ + "versions.yml:md5,384c5398cae017e4fe0df8cb8f004017" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.1" + }, + "timestamp": "2024-05-21T10:48:47.945706955" + } +} \ No newline at end of file diff --git a/modules/nf-core/fgbio/sortbam/tests/tags.yml b/modules/nf-core/fgbio/sortbam/tests/tags.yml new file mode 100644 index 0000000..da42eab --- /dev/null +++ b/modules/nf-core/fgbio/sortbam/tests/tags.yml @@ -0,0 +1,2 @@ +fgbio/sortbam: + - "modules/nf-core/fgbio/sortbam/**" diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index ca39fb6..a31464c 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.21 + - bioconda::multiqc=1.24.1 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 47ac352..d536580 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,14 +3,16 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : - 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.24.1--pyhdfd78af_0' : + 'biocontainers/multiqc:1.24.1--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" path(multiqc_config) path(extra_multiqc_config) path(multiqc_logo) + path(replace_names) + path(sample_names) output: path "*multiqc_report.html", emit: report @@ -23,16 +25,22 @@ process MULTIQC { script: def args = task.ext.args ?: '' + def prefix = task.ext.prefix ? "--filename ${task.ext.prefix}.html" : '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' + def replace = replace_names ? "--replace-names ${replace_names}" : '' + def samples = sample_names ? "--sample-names ${sample_names}" : '' """ multiqc \\ --force \\ $args \\ $config \\ + $prefix \\ $extra_config \\ $logo \\ + $replace \\ + $samples \\ . cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index 45a9bc3..382c08c 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -29,6 +29,19 @@ input: type: file description: Optional logo file for MultiQC pattern: "*.{png}" + - replace_names: + type: file + description: | + Optional two-column sample renaming file. First column a set of + patterns, second column a set of corresponding replacements. Passed via + MultiQC's `--replace-names` option. + pattern: "*.{tsv}" + - sample_names: + type: file + description: | + Optional TSV file with headers, passed to the MultiQC --sample_names + argument. + pattern: "*.{tsv}" output: - report: type: file diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index f1c4242..33316a7 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -8,6 +8,8 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" + config "./nextflow.config" + test("sarscov2 single-end [fastqc]") { when { @@ -17,6 +19,8 @@ nextflow_process { input[1] = [] input[2] = [] input[3] = [] + input[4] = [] + input[5] = [] """ } } @@ -41,6 +45,8 @@ nextflow_process { input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] + input[4] = [] + input[5] = [] """ } } @@ -66,6 +72,8 @@ nextflow_process { input[1] = [] input[2] = [] input[3] = [] + input[4] = [] + input[5] = [] """ } } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index bfebd80..83fa080 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.2" }, - "timestamp": "2024-02-29T08:48:55.657331" + "timestamp": "2024-07-10T12:41:34.562023" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.2" }, - "timestamp": "2024-02-29T08:49:49.071937" + "timestamp": "2024-07-10T11:27:11.933869532" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + "versions.yml:md5,6eb13f3b11bbcbfc98ad3166420ff760" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.2" }, - "timestamp": "2024-02-29T08:49:25.457567" + "timestamp": "2024-07-10T11:26:56.709849369" } -} \ No newline at end of file +} diff --git a/modules/nf-core/multiqc/tests/nextflow.config b/modules/nf-core/multiqc/tests/nextflow.config new file mode 100644 index 0000000..c537a6a --- /dev/null +++ b/modules/nf-core/multiqc/tests/nextflow.config @@ -0,0 +1,5 @@ +process { + withName: 'MULTIQC' { + ext.prefix = null + } +} diff --git a/modules/nf-core/samtools/dict/tests/main.nf.test b/modules/nf-core/samtools/dict/tests/main.nf.test new file mode 100644 index 0000000..c467081 --- /dev/null +++ b/modules/nf-core/samtools/dict/tests/main.nf.test @@ -0,0 +1,62 @@ +nextflow_process { + + name "Test Process SAMTOOLS_DICT" + script "../main.nf" + process "SAMTOOLS_DICT" + + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/dict" + + test("sarscov2 - fasta") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + path(process.out.dict[0][1]).readLines()[0], + process.out.versions + ).match() + } + ) + } + + } + + test("sarscov2 - fasta - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/samtools/dict/tests/main.nf.test.snap b/modules/nf-core/samtools/dict/tests/main.nf.test.snap new file mode 100644 index 0000000..66d7b1f --- /dev/null +++ b/modules/nf-core/samtools/dict/tests/main.nf.test.snap @@ -0,0 +1,50 @@ +{ + "sarscov2 - fasta - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "genome.fasta.dict:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,47f9e889ed1e779cb54e61d707a7135d" + ], + "dict": [ + [ + { + "id": "test", + "single_end": false + }, + "genome.fasta.dict:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,47f9e889ed1e779cb54e61d707a7135d" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-18T09:43:30.509703257" + }, + "sarscov2 - fasta": { + "content": [ + "@HD\tVN:1.0\tSO:unsorted", + [ + "versions.yml:md5,0af6b2d2d6d9d96bc5abe98b99b91446" + ] + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-30T12:12:31.201483952" + } +} \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index ef0d178..7702358 100644 --- a/nextflow.config +++ b/nextflow.config @@ -19,11 +19,13 @@ params { save_reference = false // MultiQC options - multiqc_config = null - multiqc_title = null - multiqc_logo = null - max_multiqc_email_size = '25.MB' + multiqc_config = null + multiqc_title = null + multiqc_logo = null + max_multiqc_email_size = '25.MB' multiqc_methods_description = null + multiqc_replace_names = null + multiqc_sample_names = null // Main options mode = 'rd' diff --git a/nextflow_schema.json b/nextflow_schema.json index 90aa755..dc54a5c 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -354,6 +354,20 @@ "description": "Custom MultiQC yaml file containing HTML including a methods description.", "fa_icon": "fas fa-cog" }, + "multiqc_replace_names": { + "type": "string", + "format": "file-path", + "description": "Two-column sample renaming TSV file passed to MultiQC. First column a set of patterns, second column a set of corresponding replacements.", + "fa_icon": "fas fa-cog", + "hidden": true + }, + "multiqc_sample_names": { + "type": "string", + "format": "file-path", + "description": " TSV file with headers passed to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true + }, "validate_params": { "type": "boolean", "description": "Boolean whether to validate parameters against the schema at runtime", diff --git a/workflows/fastquorum.nf b/workflows/fastquorum.nf index 5f69526..0b79c10 100644 --- a/workflows/fastquorum.nf +++ b/workflows/fastquorum.nf @@ -194,6 +194,9 @@ workflow FASTQUORUM { ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + ch_replace_names = params.multiqc_replace_names ? Channel.fromPath(params.multiqc_replace_names, checkIfExists: true): Channel.empty() + ch_sample_names = params.multiqc_sample_names ? Channel.fromPath(params.multiqc_sample_names, checkIfExists: true): Channel.empty() summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) @@ -206,7 +209,9 @@ workflow FASTQUORUM { ch_multiqc_files.collect(), ch_multiqc_config.toList(), ch_multiqc_custom_config.toList(), - ch_multiqc_logo.toList() + ch_multiqc_logo.toList(), + ch_replace_names.toList(), + ch_sample_names.toList() ) emit: From 513660bf304e3e871ced943a9b881d204ab85051 Mon Sep 17 00:00:00 2001 From: znorgaard Date: Fri, 23 Aug 2024 09:34:37 -0700 Subject: [PATCH 25/30] chore: update subworkflow --- subworkflows/nf-core/utils_nextflow_pipeline/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf index ac31f28..e770d91 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -102,7 +102,7 @@ def checkCondaChannels() { // Check that all channels are present // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def required_channels_in_order = ['conda-forge', 'bioconda'] def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean // Check that they are in the right order From 0d5f280feca3b6381298bc0a6105e66fc803fc9d Mon Sep 17 00:00:00 2001 From: znorgaard Date: Fri, 23 Aug 2024 12:42:43 -0700 Subject: [PATCH 26/30] chore: update snapshots --- modules/nf-core/fastqc/tests/main.nf.test.snap | 12 ++++++------ tests/pipeline/multi_fastq.nf.test.snap | 14 +++++++------- tests/pipeline/multi_lanes.nf.test.snap | 14 +++++++------- tests/pipeline/single_fastq.nf.test.snap | 14 +++++++------- tests/pipeline/tiny.nf.test.snap | 14 +++++++------- 5 files changed, 34 insertions(+), 34 deletions(-) diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index d5db309..8067d2b 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -156,14 +156,14 @@ "sarscov2 paired-end [fastq]": { "content": [ [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.04.3" + "nextflow": "24.04.4" }, - "timestamp": "2024-07-22T11:01:31.188871" + "timestamp": "2024-08-23T11:21:58.323262" }, "sarscov2 paired-end [fastq] - stub": { "content": [ @@ -274,14 +274,14 @@ "sarscov2 single-end [fastq]": { "content": [ [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.04.3" + "nextflow": "24.04.4" }, - "timestamp": "2024-07-22T11:01:19.095607" + "timestamp": "2024-08-23T11:21:51.69768" }, "sarscov2 interleaved [fastq] - stub": { "content": [ diff --git a/tests/pipeline/multi_fastq.nf.test.snap b/tests/pipeline/multi_fastq.nf.test.snap index 73b9ee0..cfa57e4 100644 --- a/tests/pipeline/multi_fastq.nf.test.snap +++ b/tests/pipeline/multi_fastq.nf.test.snap @@ -16,23 +16,23 @@ }, "multi_fastq_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1}}" ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.4" }, - "timestamp": "2024-05-20T16:13:43.042208" + "timestamp": "2024-08-23T11:52:45.734188" }, "multi_fastq_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1}}" ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.4" }, - "timestamp": "2024-05-20T16:06:43.154704" + "timestamp": "2024-08-23T11:50:06.73123" }, "multi_fastq_ht": { "content": [ @@ -49,4 +49,4 @@ }, "timestamp": "2024-05-20T01:58:10.241449" } -} +} \ No newline at end of file diff --git a/tests/pipeline/multi_lanes.nf.test.snap b/tests/pipeline/multi_lanes.nf.test.snap index 156c3cd..efba1a7 100644 --- a/tests/pipeline/multi_lanes.nf.test.snap +++ b/tests/pipeline/multi_lanes.nf.test.snap @@ -1,13 +1,13 @@ { "multi_lanes_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, SORTBAM={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.2}, SORTBAM={fgbio=2.2.1}, Workflow={nf-core/fastquorum=v1.0.1}}" ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.4" }, - "timestamp": "2024-05-30T00:51:13.471176" + "timestamp": "2024-08-23T11:44:11.374426" }, "multi_lanes_ht": { "content": [ @@ -26,13 +26,13 @@ }, "multi_lanes_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.19.2}, SORTBAM={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, MERGE_BAM={samtools=1.2}, SORTBAM={fgbio=2.2.1}, Workflow={nf-core/fastquorum=v1.0.1}}" ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.4" }, - "timestamp": "2024-05-30T00:59:50.302089" + "timestamp": "2024-08-23T11:47:27.269353" }, "multi_lanes_rd": { "content": [ @@ -49,4 +49,4 @@ }, "timestamp": "2024-05-20T01:34:44.993842" } -} +} \ No newline at end of file diff --git a/tests/pipeline/single_fastq.nf.test.snap b/tests/pipeline/single_fastq.nf.test.snap index 2b1e42f..d676ab8 100644 --- a/tests/pipeline/single_fastq.nf.test.snap +++ b/tests/pipeline/single_fastq.nf.test.snap @@ -11,23 +11,23 @@ }, "single_fastq_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1}}" ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.4" }, - "timestamp": "2024-05-20T16:28:01.840894" + "timestamp": "2024-08-23T11:56:22.904524" }, "single_fastq_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLMOLECULARCONSENSUSREADS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1}}" ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.4" }, - "timestamp": "2024-05-20T16:21:29.23891" + "timestamp": "2024-08-23T11:54:37.217511" }, "single_fastq_ht": { "content": [ @@ -39,4 +39,4 @@ }, "timestamp": "2024-05-20T07:39:38.166122" } -} +} \ No newline at end of file diff --git a/tests/pipeline/tiny.nf.test.snap b/tests/pipeline/tiny.nf.test.snap index 7718da2..e6a32c5 100644 --- a/tests/pipeline/tiny.nf.test.snap +++ b/tests/pipeline/tiny.nf.test.snap @@ -1,23 +1,23 @@ { "tiny_rd_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLDDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, FILTERCONSENSUSREADS={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1}}" ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.4" }, - "timestamp": "2024-05-20T15:35:22.555322" + "timestamp": "2024-08-23T11:38:48.555493" }, "tiny_ht_software_versions": { "content": [ - "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=1.0.1}}" + "{ALIGN_CONSENSUS_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, ALIGN_RAW_BAM={bwa=0.7.17-r1188, fgbio=2.0.2, samtools=1.16.1}, CALLANDFILTERDUPLEXCONSENSUSREADS={fgbio=2.0.2}, COLLECTDUPLEXSEQMETRICS={fgbio=2.0.2}, FASTQC={fastqc=0.12.1}, FASTQTOBAM={fgbio=2.0.2}, GROUPREADSBYUMI={fgbio=2.0.2}, Workflow={nf-core/fastquorum=v1.0.1}}" ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.4" }, - "timestamp": "2024-05-20T15:42:18.622029" + "timestamp": "2024-08-23T11:40:38.676264" }, "tiny_rd": { "content": [ @@ -41,4 +41,4 @@ }, "timestamp": "2024-05-19T23:48:57.112733" } -} +} \ No newline at end of file From edd8685376ce98d5e4ba91bec3924a1e16ae47f8 Mon Sep 17 00:00:00 2001 From: znorgaard Date: Wed, 4 Sep 2024 13:05:55 -0700 Subject: [PATCH 27/30] chore: finalize release date --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 1580d5f..65d72f7 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## [[1.0.1]](https://github.com/nf-core/fastquorum/releases/tag/1.0.1) -- 2024-08-?? +## [[1.0.1]](https://github.com/nf-core/fastquorum/releases/tag/1.0.1) -- 2024-09-04 ### Credits From 75e6803ad60e08962e8fea8b4c9f23ae8dd753ed Mon Sep 17 00:00:00 2001 From: Simon Pearce <24893913+SPPearce@users.noreply.github.com> Date: Thu, 5 Sep 2024 05:45:45 +0000 Subject: [PATCH 28/30] Add stubs to all modules --- CHANGELOG.md | 1 + modules/local/align_bam/main.nf | 16 ++++++++++++++++ .../callandfilterduplexconsensusreads/main.nf | 11 +++++++++++ .../callandfiltermolecularconsensusreads/main.nf | 11 +++++++++++ .../local/fgbio/callduplexconsensusreads/main.nf | 11 +++++++++++ .../fgbio/callmolecularconsensusreads/main.nf | 11 +++++++++++ .../local/fgbio/collectduplexseqmetrics/main.nf | 15 +++++++++++++++ modules/local/fgbio/fastqtobam/main.nf | 11 +++++++++++ modules/local/fgbio/filterconsensusreads/main.nf | 14 +++++++++++++- modules/local/fgbio/groupreadsbyumi/main.nf | 12 ++++++++++++ 10 files changed, 112 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 65d72f7..b108d95 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -20,6 +20,7 @@ Special thanks to the following for their contributions to the release: - [PR #60](https://github.com/nf-core/fastquorum/pull/60) - Enable automatic escalation of memory for FilterConsensusReads. - [PR #67](https://github.com/nf-core/fastquorum/pull/67) - Fix setting the parameters from igenomes. - [PR #68](https://github.com/nf-core/fastquorum/pull/68) - Temporary fix to merging BAMs across lanes in template-coordinate order. Using `fgbio SortBam` after `samtools merge`. Related to [samtools#2062](https://github.com/samtools/samtools/pull/2062). +- [PR #71](https://github.com/nf-core/fastquorum/pull/71) - Add stubs to all local modules. ## [[1.0.0]](https://github.com/nf-core/fastquorum/releases/tag/1.0.0) -- 2024-05-20 diff --git a/modules/local/align_bam/main.nf b/modules/local/align_bam/main.nf index 6a298f5..f4cfb0f 100644 --- a/modules/local/align_bam/main.nf +++ b/modules/local/align_bam/main.nf @@ -89,4 +89,20 @@ process ALIGN_BAM { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def index_command = (sort_type != "template-coordinate") ? "touch ${prefix}.mapped.bam.bai" : "" + """ + touch ${prefix}.mapped.bam + $index_command + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') + fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + } diff --git a/modules/local/fgbio/callandfilterduplexconsensusreads/main.nf b/modules/local/fgbio/callandfilterduplexconsensusreads/main.nf index 8a78bc6..32d9be1 100644 --- a/modules/local/fgbio/callandfilterduplexconsensusreads/main.nf +++ b/modules/local/fgbio/callandfilterduplexconsensusreads/main.nf @@ -68,4 +68,15 @@ process FGBIO_CALLANDFILTERDUPLEXCONSENSUSREADS { fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.cons.filtered.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') + END_VERSIONS + """ } diff --git a/modules/local/fgbio/callandfiltermolecularconsensusreads/main.nf b/modules/local/fgbio/callandfiltermolecularconsensusreads/main.nf index 899fcf3..9fedadb 100644 --- a/modules/local/fgbio/callandfiltermolecularconsensusreads/main.nf +++ b/modules/local/fgbio/callandfiltermolecularconsensusreads/main.nf @@ -68,4 +68,15 @@ process FGBIO_CALLANDFILTERMOLECULARCONSENSUSREADS { fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.cons.filtered.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') + END_VERSIONS + """ } diff --git a/modules/local/fgbio/callduplexconsensusreads/main.nf b/modules/local/fgbio/callduplexconsensusreads/main.nf index 73a234c..939065d 100644 --- a/modules/local/fgbio/callduplexconsensusreads/main.nf +++ b/modules/local/fgbio/callduplexconsensusreads/main.nf @@ -45,4 +45,15 @@ process FGBIO_CALLDDUPLEXCONSENSUSREADS { fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.cons.unmapped.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') + END_VERSIONS + """ } diff --git a/modules/local/fgbio/callmolecularconsensusreads/main.nf b/modules/local/fgbio/callmolecularconsensusreads/main.nf index 3c00c47..6bf303d 100644 --- a/modules/local/fgbio/callmolecularconsensusreads/main.nf +++ b/modules/local/fgbio/callmolecularconsensusreads/main.nf @@ -45,4 +45,15 @@ process FGBIO_CALLMOLECULARCONSENSUSREADS { fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.cons.unmapped.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') + END_VERSIONS + """ } diff --git a/modules/local/fgbio/collectduplexseqmetrics/main.nf b/modules/local/fgbio/collectduplexseqmetrics/main.nf index d479366..cf9e063 100644 --- a/modules/local/fgbio/collectduplexseqmetrics/main.nf +++ b/modules/local/fgbio/collectduplexseqmetrics/main.nf @@ -37,6 +37,21 @@ process FGBIO_COLLECTDUPLEXSEQMETRICS { --duplex-umi-counts=true \\ $args; + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') + END_VERSIONS + """ + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.duplex_seq_metrics.duplex_family_sizes.txt + touch ${prefix}.duplex_seq_metrics.duplex_umi_counts.txt + touch ${prefix}.duplex_seq_metrics.duplex_yield_metrics.txt + touch ${prefix}.duplex_seq_metrics.family_sizes.txt + touch ${prefix}.duplex_seq_metrics.umi_counts.txt + touch ${prefix}.duplex_seq_metrics.duplex_qc.pdf + cat <<-END_VERSIONS > versions.yml "${task.process}": fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') diff --git a/modules/local/fgbio/fastqtobam/main.nf b/modules/local/fgbio/fastqtobam/main.nf index 346de71..543b97f 100644 --- a/modules/local/fgbio/fastqtobam/main.nf +++ b/modules/local/fgbio/fastqtobam/main.nf @@ -47,4 +47,15 @@ process FGBIO_FASTQTOBAM { fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.unmapped.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') + END_VERSIONS + """ } diff --git a/modules/local/fgbio/filterconsensusreads/main.nf b/modules/local/fgbio/filterconsensusreads/main.nf index 118fab6..6943765 100644 --- a/modules/local/fgbio/filterconsensusreads/main.nf +++ b/modules/local/fgbio/filterconsensusreads/main.nf @@ -16,7 +16,7 @@ process FGBIO_FILTERCONSENSUSREADS { output: tuple val(meta), path("*.cons.filtered.bam") , emit: bam - tuple val(meta), path("*.cons.filtered.bam.bai") , emit: bai + tuple val(meta), path("*.cons.filtered.bam.bai") , emit: bai path "versions.yml" , emit: versions script: @@ -54,4 +54,16 @@ process FGBIO_FILTERCONSENSUSREADS { fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.cons.filtered.bam + touch ${prefix}.cons.filtered.bam.bai + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') + END_VERSIONS + """ } diff --git a/modules/local/fgbio/groupreadsbyumi/main.nf b/modules/local/fgbio/groupreadsbyumi/main.nf index 8eaa544..e650599 100644 --- a/modules/local/fgbio/groupreadsbyumi/main.nf +++ b/modules/local/fgbio/groupreadsbyumi/main.nf @@ -46,4 +46,16 @@ process FGBIO_GROUPREADSBYUMI { fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.grouped.bam + touch ${prefix}.grouped-family-sizes.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') + END_VERSIONS + """ } From be54c8f2ffd37e19b050f9c7725f16b25df9d5f1 Mon Sep 17 00:00:00 2001 From: Simon Pearce <24893913+SPPearce@users.noreply.github.com> Date: Thu, 5 Sep 2024 20:01:01 +0100 Subject: [PATCH 29/30] Update modules/local/fgbio/collectduplexseqmetrics/main.nf --- modules/local/fgbio/collectduplexseqmetrics/main.nf | 1 + 1 file changed, 1 insertion(+) diff --git a/modules/local/fgbio/collectduplexseqmetrics/main.nf b/modules/local/fgbio/collectduplexseqmetrics/main.nf index cf9e063..4675898 100644 --- a/modules/local/fgbio/collectduplexseqmetrics/main.nf +++ b/modules/local/fgbio/collectduplexseqmetrics/main.nf @@ -42,6 +42,7 @@ process FGBIO_COLLECTDUPLEXSEQMETRICS { fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//') END_VERSIONS """ + stub: def prefix = task.ext.prefix ?: "${meta.id}" """ From a5fc799867eddc9926bd49eba800cbea502c97b6 Mon Sep 17 00:00:00 2001 From: znorgaard Date: Tue, 10 Sep 2024 08:39:11 -0700 Subject: [PATCH 30/30] chore: correct date --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index b108d95..1a3a9f9 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## [[1.0.1]](https://github.com/nf-core/fastquorum/releases/tag/1.0.1) -- 2024-09-04 +## [[1.0.1]](https://github.com/nf-core/fastquorum/releases/tag/1.0.1) -- 2024-09-10 ### Credits