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In the rnaSPAdes configuration when setting the pipeline up, there is some text that says:
Set strand-specific type for rnaSPAdes. Use `--ss rf` when first read in pair corresponds to reverse gene strand (antisense data, e.g. obtained via dUTP protocol) and `--ss fr` otherwise (forward).
To me, this suggests that you should enter --ss rf when configuring that parameter, however this results in the error below. The parameter entered here should just be rf or fr. See screenshot below to illustrate where we went wrong...
spades.py: error: argument --ss: expected one argument
I would suggest clarifying the text by changing "Use --ss rf when first read in pair..." to be "Use rf when first read in pair"
Command used and terminal output
Relevant files
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The text was updated successfully, but these errors were encountered:
Apologies for the confusion. You would only need to type rf into that field, and it will get interpreted as --ss rf when running the pipeline.
I will update the instructions to clarify
Description of the bug
In the rnaSPAdes configuration when setting the pipeline up, there is some text that says:
To me, this suggests that you should enter
--ss rf
when configuring that parameter, however this results in the error below. The parameter entered here should just berf
orfr
. See screenshot below to illustrate where we went wrong...I would suggest clarifying the text by changing "Use --ss rf when first read in pair..." to be "Use rf when first read in pair"
Command used and terminal output
Relevant files
No response
System information
No response
The text was updated successfully, but these errors were encountered: