First, install the tool using pip.
pip install affipred
Provide input and output files with relative or absolute path:
affipred variants.vcf -o affipred_results.csv
The input should be a .vcf file while the output file name extension should be .csv.
The output file will contain all the features used to predict the impact of the variants alongside the AFFIPred scores and the prediction of functional impact of the variants.