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While SplitsTree4 was designed for unrooted phylogenetic trees and networks, another previous program Dendroscope3 [(Huson and Scornavacca, 2012)](https://academic.oup.com/sysbio/article/61/6/1061/1666897) was designed for working with rooted trees and networks. This program, too, was designed for much smaller datasets than people work with today.
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## Rooted networks
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Starting with release 6.5.0, SplitsTree will be using new algorithms (including OD optimization) and code for visualizing rooted phylogentic networks, both in a combining view and a transfer view, as described here:
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[(Huson, 2025)](https://www.biorxiv.org/content/10.1101/2025.04.01.646633v1).
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## How to cite
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If you use SplitsTree App, the please cite this paper:

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