diff --git a/src/webgpu/api/operation/rendering/depth_bias.spec.ts b/src/webgpu/api/operation/rendering/depth_bias.spec.ts
index 08fdb85df687..cf7eace6e7eb 100644
--- a/src/webgpu/api/operation/rendering/depth_bias.spec.ts
+++ b/src/webgpu/api/operation/rendering/depth_bias.spec.ts
@@ -5,7 +5,11 @@ Tests render results with different depth bias values like 'positive', 'negative
 
 import { makeTestGroup } from '../../../../common/framework/test_group.js';
 import { unreachable } from '../../../../common/util/util.js';
-import { DepthStencilFormat, EncodableTextureFormat } from '../../../capability_info.js';
+import {
+  DepthStencilFormat,
+  EncodableTextureFormat,
+  kTextureFormatInfo,
+} from '../../../capability_info.js';
 import { GPUTest } from '../../../gpu_test.js';
 import { kValue } from '../../../util/constants.js';
 import { TexelView } from '../../../util/texture/texel_view.js';
@@ -28,23 +32,25 @@ enum QuadAngle {
 const kPointTwoFiveBiasForPointTwoFiveZOnFloat = 8388608;
 
 class DepthBiasTest extends GPUTest {
-  runDepthBiasTest(
-    depthFormat: EncodableTextureFormat & DepthStencilFormat,
+  runDepthBiasTestInternal(
+    depthFormat: DepthStencilFormat,
     {
       quadAngle,
       bias,
       biasSlopeScale,
       biasClamp,
-      expectedDepth,
+      initialDepth,
     }: {
       quadAngle: QuadAngle;
       bias: number;
       biasSlopeScale: number;
       biasClamp: number;
-      expectedDepth: number;
+      initialDepth: number;
     }
-  ) {
+  ): { renderTarget: GPUTexture; depthTexture: GPUTexture } {
     const renderTargetFormat = 'rgba8unorm';
+    const depthFormatInfo = kTextureFormatInfo[depthFormat];
+
     let vertexShaderCode: string;
     switch (quadAngle) {
       case QuadAngle.Flat:
@@ -99,10 +105,11 @@ class DepthBiasTest extends GPUTest {
 
     const depthStencilAttachment: GPURenderPassDepthStencilAttachment = {
       view: depthTexture.createView(),
-      depthLoadOp: 'load',
-      depthStoreOp: 'store',
-      stencilLoadOp: 'load',
-      stencilStoreOp: 'store',
+      depthLoadOp: depthFormatInfo.depth ? 'clear' : undefined,
+      depthStoreOp: depthFormatInfo.depth ? 'store' : undefined,
+      stencilLoadOp: depthFormatInfo.stencil ? 'clear' : undefined,
+      stencilStoreOp: depthFormatInfo.stencil ? 'store' : undefined,
+      depthClearValue: initialDepth,
     };
 
     const encoder = this.device.createCommandEncoder();
@@ -138,6 +145,33 @@ class DepthBiasTest extends GPUTest {
     pass.end();
     this.device.queue.submit([encoder.finish()]);
 
+    return { renderTarget, depthTexture };
+  }
+
+  runDepthBiasTest(
+    depthFormat: EncodableTextureFormat & DepthStencilFormat,
+    {
+      quadAngle,
+      bias,
+      biasSlopeScale,
+      biasClamp,
+      expectedDepth,
+    }: {
+      quadAngle: QuadAngle;
+      bias: number;
+      biasSlopeScale: number;
+      biasClamp: number;
+      expectedDepth: number;
+    }
+  ) {
+    const { renderTarget, depthTexture } = this.runDepthBiasTestInternal(depthFormat, {
+      quadAngle,
+      bias,
+      biasSlopeScale,
+      biasClamp,
+      initialDepth: 0,
+    });
+
     const expColor = { Depth: expectedDepth };
     const expTexelView = TexelView.fromTexelsAsColors(depthFormat, coords => expColor);
 
@@ -150,6 +184,52 @@ class DepthBiasTest extends GPUTest {
     );
     this.eventualExpectOK(result);
     this.trackForCleanup(renderTarget);
+    this.trackForCleanup(depthTexture);
+  }
+
+  runDepthBiasTestFor24BitFormat(
+    depthFormat: DepthStencilFormat,
+    {
+      quadAngle,
+      bias,
+      biasSlopeScale,
+      biasClamp,
+      expectedColor,
+    }: {
+      quadAngle: QuadAngle;
+      bias: number;
+      biasSlopeScale: number;
+      biasClamp: number;
+      expectedColor: Float32Array;
+    }
+  ) {
+    const { renderTarget, depthTexture } = this.runDepthBiasTestInternal(depthFormat, {
+      quadAngle,
+      bias,
+      biasSlopeScale,
+      biasClamp,
+      initialDepth: 0.4,
+    });
+
+    const renderTargetFormat = 'rgba8unorm';
+    const expColor = {
+      R: expectedColor[0],
+      G: expectedColor[1],
+      B: expectedColor[2],
+      A: expectedColor[3],
+    };
+    const expTexelView = TexelView.fromTexelsAsColors(renderTargetFormat, coords => expColor);
+
+    const result = textureContentIsOKByT2B(
+      this,
+      { texture: renderTarget },
+      [1, 1],
+      { expTexelView },
+      { maxDiffULPsForNormFormat: 1 }
+    );
+    this.eventualExpectOK(result);
+    this.trackForCleanup(renderTarget);
+    this.trackForCleanup(depthTexture);
   }
 
   createRenderPipelineForTest(
@@ -186,8 +266,6 @@ g.test('depth_bias')
     `
   Tests that a square with different depth bias values like 'positive', 'negative', 'infinity',
   'slope', 'clamp', etc. is drawn as expected.
-
-  TODO: Need to test 'depth24plus-stencil8' format?
   `
   )
   .params(u =>
@@ -261,3 +339,46 @@ g.test('depth_bias')
   .fn(async t => {
     t.runDepthBiasTest('depth32float', t.params);
   });
+
+g.test('depth_bias_24bit_format')
+  .desc(
+    `
+  Tests that a square with different depth bias values like 'positive', 'negative',
+  'slope', 'clamp', etc. is drawn as expected with 24 bit depth format.
+
+  TODO: Enhance these tests by reading back the depth (emulating the copy using texture sampling)
+  and checking the result directly, like the non-24-bit depth tests, instead of just relying on
+  whether the depth test passes or fails.
+  `
+  )
+  .params(u =>
+    u //
+      .combine('format', ['depth24plus', 'depth24plus-stencil8'] as const)
+      .combineWithParams([
+        {
+          quadAngle: QuadAngle.Flat,
+          bias: 0.25 * (1 << 25),
+          biasSlopeScale: 0,
+          biasClamp: 0,
+          expectedColor: new Float32Array([1.0, 0.0, 0.0, 1.0]),
+        },
+        {
+          quadAngle: QuadAngle.TiltedX,
+          bias: 0.25 * (1 << 25),
+          biasSlopeScale: 1,
+          biasClamp: 0,
+          expectedColor: new Float32Array([1.0, 0.0, 0.0, 1.0]),
+        },
+        {
+          quadAngle: QuadAngle.Flat,
+          bias: 0.25 * (1 << 25),
+          biasSlopeScale: 0,
+          biasClamp: 0.1,
+          expectedColor: new Float32Array([0.0, 0.0, 0.0, 0.0]),
+        },
+      ] as const)
+  )
+  .fn(async t => {
+    const { format } = t.params;
+    t.runDepthBiasTestFor24BitFormat(format, t.params);
+  });