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Mitchell R. Vollger
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src/quick-start.md

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# Fiber-seq starting with PacBio
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For using Fiber-seq data it is important to **check with your sequencing provider prior to sequencing** to ensure that the output file will have the required information for Fiber-seq. The provider must select for the instrument to generate m6A calls using `jasmine` on instrument (if using SPQR chemistry or latter) or for the output to include average kinetics information in the CCS BAM (if using earlier chemistries), without this information `fibertools` will not be able to make m6A calls.
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### Predict m6A and infer nucleosomes
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#### Your PacBio data uses the SPQR chemistry or latter

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