Skip to content

Commit 6025724

Browse files
author
Mitchell R. Vollger
committed
expand ONT docs
1 parent 9b2ac3d commit 6025724

1 file changed

Lines changed: 2 additions & 2 deletions

File tree

src/quick-start.md

Lines changed: 2 additions & 2 deletions
Original file line numberDiff line numberDiff line change
@@ -58,8 +58,6 @@ You can either use [Dorado](https://github.com/nanoporetech/dorado) to align you
5858

5959
If you do want to do phasing we recommend using [WhatsHap](https://whatshap.readthedocs.io/en/latest/) for phasing ONT data. Please see their documentation for more information.
6060

61-
After this point, you will have a Fiber-seq BAM file that is compatible with all the [extraction](fibertools/extracting/extracting.md) commands in `fibertools`.
62-
6361
### A full example for processing ONT data
6462

6563
Here is an example summary of the commands to process ONT data assuming you have already completed 6mA and CpG calling with `dorado`:
@@ -77,6 +75,8 @@ modkit call-mods -p 0.1 ONT.dorado.with.6mA.bam - \
7775
| samtools sort -@ 32 --write-index -o ont.fiberseq.bam
7876
```
7977

78+
After this point, you will have a Fiber-seq BAM file that is compatible with all the [extraction](fibertools/extracting/extracting.md) commands in `fibertools`.
79+
8080
### Fiber-seq peaks and UCSC browser tracks
8181

8282
We have had good success in applying the [FIRE pipeline](https://github.com/fiberseq/FIRE) to ONT data. However, this does require a heuristic in FIRE that must be enabled. To enable this add `ont: true` to your `config.yaml` file when setting up your FIRE run.

0 commit comments

Comments
 (0)