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Mitchell R. Vollger
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expand ONT docs
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src/quick-start.md

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@@ -50,14 +50,12 @@ If you do use Dorado you must then filter the m6A calls with [modkit](https://gi
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modkit call-mods -t 8 -p 0.1 input.dorado.bam filtered.dorado.bam
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```
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We also show how to apply this filter and call nucleosomes in one line in the next section.
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### Infer nucleosomes and MSPs
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Once you have CpG and m6A information in your filtered ONT BAM file, you can use [`ft add-nucleosomes`](fibertools/help.md#ft-add-nucleosomes) to infer nucleosomes and MSPs. With Dorado, we find the best results when restricting to the 90% of calls that `dorado` is most confident in as determined by [modkit](https://github.com/nanoporetech/modkit).
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Once you have CpG and m6A information in your filtered ONT BAM file, you can use [`ft add-nucleosomes`](fibertools/help.md#ft-add-nucleosomes) to infer nucleosomes and MSPs.
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```bash
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modkit call-mods -p 0.1 input.bam - | ft add-nucleosomes - output.bam
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ft add-nucleosomes filtered.dorado.bam output.bam
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```
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### A full example for processing ONT data

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