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iprscan5.pl
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#!/usr/bin/env perl
=head1 NAME
iprscan5.pl
=head1 DESCRIPTION
InterProScan 5 (REST) web service Perl client using L<LWP>.
Tested with:
=over
=item *
L<LWP> 6.35, L<XML::Simple> 2.25 and Perl 5.22.0 (MacOS 10.13.6)
=back
For further information see:
=over
=item *
L<https://www.ebi.ac.uk/Tools/webservices/>
=back
=head1 LICENSE
Copyright 2012-2024 EMBL - European Bioinformatics Institute
Licensed under the Apache License, Version 2.0 (the "License");
you may not use this file except in compliance with the License.
You may obtain a copy of the License at
http://www.apache.org/licenses/LICENSE-2.0
Unless required by applicable law or agreed to in writing, software
distributed under the License is distributed on an "AS IS" BASIS,
WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
See the License for the specific language governing permissions and
limitations under the License.
Perl Client Automatically generated with:
https://github.com/ebi-jdispatcher/webservice-clients-generator
=cut
# ======================================================================
# Enable Perl warnings
use strict;
use warnings;
# Load libraries
use English;
use LWP;
use XML::Simple;
use Getopt::Long qw(:config no_ignore_case bundling);
use File::Basename;
use Data::Dumper;
use Time::HiRes qw(usleep);
# Base URL for service
my $baseUrl = 'https://www.ebi.ac.uk/Tools/services/rest/iprscan5';
my $version = '2024-07-12 11:05';
# Set interval for checking status
my $checkInterval = 3;
# Set maximum number of 'ERROR' status calls to call job failed.
my $maxErrorStatusCount = 3;
# Output level
my $outputLevel = 1;
# Process command-line options
my $numOpts = scalar(@ARGV);
my %params = (
'debugLevel' => 0,
'maxJobs' => 1
);
# Default parameter values (should get these from the service)
GetOptions(
# Tool specific options
'goterms' => \$params{'goterms'}, # Switch on look-up of corresponding Gene Ontology annotations
'pathways' => \$params{'pathways'}, # Switch on look-up of corresponding pathway annotations
'stype=s' => \$params{'stype'}, # Indicates if the sequences to align are protein or nucleotide (DNA/RNA).
'appl=s' => \$params{'appl'}, # A number of different protein sequence applications are launched. These applications search against specific databases and have preconfigured cut off thresholds.
'sequence=s' => \$params{'sequence'}, # Your protein sequence can be entered directly into this form in GCG, FASTA, EMBL, PIR, NBRF or UniProtKB/Swiss-Prot format. A partially formatted sequence is not accepted. Adding a return to the end of the sequence may help certain applications understand the input. Note that directly using data from word processors may yield unpredictable results as hidden/control characters may be present.
# Generic options
'email=s' => \$params{'email'}, # User e-mail address
'title=s' => \$params{'title'}, # Job title
'outfile=s' => \$params{'outfile'}, # Output file name
'outformat=s' => \$params{'outformat'}, # Output file type
'jobid=s' => \$params{'jobid'}, # JobId
'help|h' => \$params{'help'}, # Usage help
'asyncjob' => \$params{'asyncjob'}, # Asynchronous submission
'polljob' => \$params{'polljob'}, # Get results
'pollFreq=f' => \$params{'pollFreq'}, # Poll Frequency
'resultTypes' => \$params{'resultTypes'}, # Get result types
'status' => \$params{'status'}, # Get status
'params' => \$params{'params'}, # List input parameters
'paramDetail=s' => \$params{'paramDetail'}, # Get details for parameter
'multifasta' => \$params{'multifasta'}, # Multiple fasta input
'useSeqId' => \$params{'useSeqId'}, # Seq Id file name
'maxJobs=i' => \$params{'maxJobs'}, # Max. parallel jobs
'verbose' => \$params{'verbose'}, # Increase output level
'version' => \$params{'version'}, # Prints out the version of the Client and exit.
'quiet' => \$params{'quiet'}, # Decrease output level
'debugLevel=i' => \$params{'debugLevel'}, # Debugging level
'baseUrl=s' => \$baseUrl, # Base URL for service.
);
if ($params{'verbose'}) {$outputLevel++}
if ($params{'quiet'}) {$outputLevel--}
if ($params{'pollFreq'}) {$checkInterval = $params{'pollFreq'} * 1000 * 1000}
if ($params{'baseUrl'}) {$baseUrl = $params{'baseUrl'}}
# Debug mode: LWP version
&print_debug_message('MAIN', 'LWP::VERSION: ' . $LWP::VERSION,
1);
# Debug mode: print the input parameters
&print_debug_message('MAIN', "params:\n" . Dumper(\%params), 11);
# LWP UserAgent for making HTTP calls (initialised when required).
my $ua;
# Get the script filename for use in usage messages
my $scriptName = basename($0, ());
# Print usage and exit if requested
if ($params{'help'} || $numOpts == 0) {
&usage();
exit(0);
}
# Debug mode: show the base URL
&print_debug_message('MAIN', 'baseUrl: ' . $baseUrl, 1);
if (
!(
$params{'polljob'}
|| $params{'resultTypes'}
|| $params{'status'}
|| $params{'params'}
|| $params{'paramDetail'}
|| $params{'version'}
)
&& !(defined($ARGV[0]) || defined($params{'sequence'}))
) {
# Bad argument combination, so print error message and usage
print STDERR 'Error: bad option combination', "\n";
&usage();
exit(1);
}
# Get parameters list
elsif ($params{'params'}) {
&print_tool_params();
}
# Get parameter details
elsif ($params{'paramDetail'}) {
&print_param_details($params{'paramDetail'});
}
# Print Client version
elsif ($params{'version'}) {
print STDOUT 'Revision: ' . $version, "\n";
exit(1);
}
# Job status
elsif ($params{'status'} && defined($params{'jobid'})) {
&print_job_status($params{'jobid'});
}
# Result types
elsif ($params{'resultTypes'} && defined($params{'jobid'})) {
&print_result_types($params{'jobid'});
}
# Poll job and get results
elsif ($params{'polljob'} && defined($params{'jobid'})) {
&get_results($params{'jobid'});
}
# Submit a job
else {
# Multiple input sequence mode, assume fasta format.
if (defined($params{'multifasta'}) && $params{'multifasta'}) {
&multi_submit_job();
}
# Entry identifier list file.
elsif ((defined($params{'sequence'}) && $params{'sequence'} =~ m/^\@/)
|| (defined($ARGV[0]) && $ARGV[0] =~ m/^\@/)) {
my $list_filename = $params{'sequence'} || $ARGV[0];
$list_filename =~ s/^\@//;
&list_file_submit_job($list_filename);
}
# Default: single sequence/identifier.
else {
# Warn for invalid batch only option use.
if (defined($params{'useSeqId'}) && $params{'useSeqId'}) {
print STDERR "Warning: --useSeqId option ignored.\n";
delete $params{'useSeqId'};
}
if (defined($params{'maxJobs'}) && $params{'maxJobs'} > 1) {
print STDERR "Warning: --maxJobs option ignored.\n";
$params{'maxJobs'} = 1;
}
# Load the sequence data and submit.
&submit_job(&load_data());
}
}
=head1 FUNCTIONS
=cut
### Wrappers for REST resources ###
=head2 rest_user_agent()
Get a LWP UserAgent to use to perform REST requests.
my $ua = &rest_user_agent();
=cut
sub rest_user_agent() {
print_debug_message('rest_user_agent', 'Begin', 21);
# Create an LWP UserAgent for making HTTP calls.
my $ua = LWP::UserAgent->new();
# Set 'User-Agent' HTTP header to identifiy the client.
my $revisionNumber = 0;
$revisionNumber = "Revision: " . $version;
$ua->agent("EBI-Sample-Client/$revisionNumber ($scriptName; $OSNAME) " . $ua->agent());
# Configure HTTP proxy support from environment.
$ua->env_proxy;
print_debug_message('rest_user_agent', 'End', 21);
return $ua;
}
=head2 rest_error()
Check a REST response for an error condition. An error is mapped to a die.
&rest_error($response, $content_data);
=cut
sub rest_error() {
print_debug_message('rest_error', 'Begin', 21);
my $response = shift;
my $contentdata;
if (scalar(@_) > 0) {
$contentdata = shift;
}
if (!defined($contentdata) || $contentdata eq '') {
$contentdata = $response->content();
}
# Check for HTTP error codes
if ($response->is_error) {
my $error_message = '';
# HTML response.
if ($contentdata =~ m/<h1>([^<]+)<\/h1>/) {
$error_message = $1;
}
# XML response.
elsif ($contentdata =~ m/<description>([^<]+)<\/description>/) {
$error_message = $1;
}
# die 'http status: ' . $response->code . ' ' . $response->message . ' ' . $error_message;
}
print_debug_message('rest_error', 'End', 21);
}
=head2 rest_request()
Perform a REST request (HTTP GET).
my $response_str = &rest_request($url);
=cut
sub rest_request {
print_debug_message('rest_request', 'Begin', 11);
my $requestUrl = shift;
print_debug_message('rest_request', 'URL: ' . $requestUrl, 11);
# Get an LWP UserAgent.
$ua = &rest_user_agent() unless defined($ua);
# Available HTTP compression methods.
my $can_accept;
eval {
$can_accept = HTTP::Message::decodable();
};
$can_accept = '' unless defined($can_accept);
# Perform the request
my $response = $ua->get($requestUrl,
'Accept-Encoding' => $can_accept, # HTTP compression.
);
print_debug_message('rest_request', 'HTTP status: ' . $response->code,
11);
print_debug_message('rest_request',
'response length: ' . length($response->content()), 11);
print_debug_message('rest_request',
'request:' . "\n" . $response->request()->as_string(), 32);
print_debug_message('rest_request',
'response: ' . "\n" . $response->as_string(), 32);
# Unpack possibly compressed response.
my $retVal;
if (defined($can_accept) && $can_accept ne '') {
$retVal = $response->decoded_content();
}
# If unable to decode use orginal content.
$retVal = $response->content() unless defined($retVal);
# Check for an error.
&rest_error($response, $retVal);
print_debug_message('rest_request', 'retVal: ' . $retVal, 12);
print_debug_message('rest_request', 'End', 11);
# Return the response data
return $retVal;
}
=head2 rest_get_parameters()
Get list of tool parameter names.
my (@param_list) = &rest_get_parameters();
=cut
sub rest_get_parameters {
print_debug_message('rest_get_parameters', 'Begin', 1);
my $url = $baseUrl . '/parameters/';
my $param_list_xml_str = rest_request($url);
my $param_list_xml = XMLin($param_list_xml_str);
my (@param_list) = @{$param_list_xml->{'id'}};
print_debug_message('rest_get_parameters', 'End', 1);
return(@param_list);
}
=head2 rest_get_parameter_details()
Get details of a tool parameter.
my $paramDetail = &rest_get_parameter_details($param_name);
=cut
sub rest_get_parameter_details {
print_debug_message('rest_get_parameter_details', 'Begin', 1);
my $parameterId = shift;
print_debug_message('rest_get_parameter_details',
'parameterId: ' . $parameterId, 1);
my $url = $baseUrl . '/parameterdetails/' . $parameterId;
my $param_detail_xml_str = rest_request($url);
my $param_detail_xml = XMLin($param_detail_xml_str);
print_debug_message('rest_get_parameter_details', 'End', 1);
return($param_detail_xml);
}
=head2 rest_run()
Submit a job.
my $job_id = &rest_run($email, $title, \%params );
=cut
sub rest_run {
print_debug_message('rest_run', 'Begin', 1);
my $email = shift;
my $title = shift;
my $params = shift;
$email = '' if (!$email);
print_debug_message('rest_run', 'email: ' . $email, 1);
if (defined($title)) {
print_debug_message('rest_run', 'title: ' . $title, 1);
}
print_debug_message('rest_run', 'params: ' . Dumper($params), 1);
# Get an LWP UserAgent.
$ua = &rest_user_agent() unless defined($ua);
# Clean up parameters
my (%tmp_params) = %{$params};
$tmp_params{'email'} = $email;
$tmp_params{'title'} = $title;
foreach my $param_name (keys(%tmp_params)) {
if (!defined($tmp_params{$param_name})) {
delete $tmp_params{$param_name};
}
}
# Submit the job as a POST
my $url = $baseUrl . '/run';
my $response = $ua->post($url, \%tmp_params);
print_debug_message('rest_run', 'HTTP status: ' . $response->code, 11);
print_debug_message('rest_run',
'request:' . "\n" . $response->request()->as_string(), 11);
print_debug_message('rest_run',
'response: ' . length($response->as_string()) . "\n" . $response->as_string(), 11);
# Check for an error.
&rest_error($response);
# The job id is returned
my $job_id = $response->content();
print_debug_message('rest_run', 'End', 1);
return $job_id;
}
=head2 rest_get_status()
Check the status of a job.
my $status = &rest_get_status($job_id);
=cut
sub rest_get_status {
print_debug_message('rest_get_status', 'Begin', 1);
my $job_id = shift;
print_debug_message('rest_get_status', 'jobid: ' . $job_id, 2);
my $status_str = 'UNKNOWN';
my $url = $baseUrl . '/status/' . $job_id;
$status_str = &rest_request($url);
print_debug_message('rest_get_status', 'status_str: ' . $status_str, 2);
print_debug_message('rest_get_status', 'End', 1);
return $status_str;
}
=head2 rest_get_result_types()
Get list of result types for finished job.
my (@result_types) = &rest_get_result_types($job_id);
=cut
sub rest_get_result_types {
print_debug_message('rest_get_result_types', 'Begin', 1);
my $job_id = shift;
print_debug_message('rest_get_result_types', 'jobid: ' . $job_id, 2);
my (@resultTypes);
my $url = $baseUrl . '/resulttypes/' . $job_id;
my $result_type_list_xml_str = &rest_request($url);
my $result_type_list_xml = XMLin($result_type_list_xml_str);
(@resultTypes) = @{$result_type_list_xml->{'type'}};
print_debug_message('rest_get_result_types',
scalar(@resultTypes) . ' result types', 2);
print_debug_message('rest_get_result_types', 'End', 1);
return(@resultTypes);
}
=head2 rest_get_result()
Get result data of a specified type for a finished job.
my $result = rest_get_result($job_id, $result_type);
=cut
sub rest_get_result {
print_debug_message('rest_get_result', 'Begin', 1);
my $job_id = shift;
my $type = shift;
print_debug_message('rest_get_result', 'jobid: ' . $job_id, 1);
print_debug_message('rest_get_result', 'type: ' . $type, 1);
my $url = $baseUrl . '/result/' . $job_id . '/' . $type;
my $result = &rest_request($url);
print_debug_message('rest_get_result', length($result) . ' characters',
1);
print_debug_message('rest_get_result', 'End', 1);
return $result;
}
### Service actions and utility functions ###
=head2 print_debug_message()
Print debug message at specified debug level.
&print_debug_message($method_name, $message, $level);
=cut
sub print_debug_message {
my $function_name = shift;
my $message = shift;
my $level = shift;
if ($level <= $params{'debugLevel'}) {
print STDERR '[', $function_name, '()] ', $message, "\n";
}
}
=head2 print_tool_params()
Print list of tool parameters.
&print_tool_params();
=cut
sub print_tool_params {
print_debug_message('print_tool_params', 'Begin', 1);
my (@param_list) = &rest_get_parameters();
foreach my $param (sort (@param_list)) {
print $param, "\n";
}
print_debug_message('print_tool_params', 'End', 1);
}
=head2 print_param_details()
Print details of a tool parameter.
&print_param_details($param_name);
=cut
sub print_param_details {
print_debug_message('print_param_details', 'Begin', 1);
my $paramName = shift;
print_debug_message('print_param_details', 'paramName: ' . $paramName, 2);
my $paramDetail = &rest_get_parameter_details($paramName);
print $paramDetail->{'name'}, "\t", $paramDetail->{'type'}, "\n";
print $paramDetail->{'description'}, "\n";
if (defined($paramDetail->{'values'}->{'value'})) {
if (ref($paramDetail->{'values'}->{'value'}) eq 'ARRAY') {
foreach my $value (@{$paramDetail->{'values'}->{'value'}}) {
&print_param_value($value);
}
}
else {
&print_param_value($paramDetail->{'values'}->{'value'});
}
}
print_debug_message('print_param_details', 'End', 1);
}
=head2 print_param_value()
Print details of a tool parameter value.
&print_param_details($param_value);
Used by print_param_details() to handle both singluar and array values.
=cut
sub print_param_value {
my $value = shift;
print $value->{'value'};
if ($value->{'defaultValue'} eq 'true') {
print "\t", 'default';
}
print "\n";
print "\t", $value->{'label'}, "\n";
if (defined($value->{'properties'})) {
foreach
my $key (sort ( keys(%{$value->{'properties'}{'property'}}) )) {
if (ref($value->{'properties'}{'property'}{$key}) eq 'HASH'
&& defined($value->{'properties'}{'property'}{$key}{'value'})
) {
print "\t", $key, "\t",
$value->{'properties'}{'property'}{$key}{'value'}, "\n";
}
else {
print "\t", $value->{'properties'}{'property'}{'key'},
"\t", $value->{'properties'}{'property'}{'value'}, "\n";
last;
}
}
}
}
=head2 print_job_status()
Print status of a job.
&print_job_status($job_id);
=cut
sub print_job_status {
print_debug_message('print_job_status', 'Begin', 1);
my $jobid = shift;
print_debug_message('print_job_status', 'jobid: ' . $jobid, 1);
if ($outputLevel > 0) {
print STDERR 'Getting status for job ', $jobid, "\n";
}
my $result = &rest_get_status($jobid);
print "$result\n";
if ($result eq 'FINISHED' && $outputLevel > 0) {
print STDERR "To get results: perl $scriptName --polljob --jobid " . $jobid
. "\n";
}
print_debug_message('print_job_status', 'End', 1);
}
=head2 print_result_types()
Print available result types for a job.
&print_result_types($job_id);
=cut
sub print_result_types {
print_debug_message('result_types', 'Begin', 1);
my $jobid = shift;
print_debug_message('result_types', 'jobid: ' . $jobid, 1);
if ($outputLevel > 0) {
print STDERR 'Getting result types for job ', $jobid, "\n";
}
my $status = &rest_get_status($jobid);
if ($status eq 'QUEUED' || $status eq 'RUNNING') {
print STDERR 'Error: Job status is ', $status,
'. To get result types the job must be finished.', "\n";
}
else {
my (@resultTypes) = &rest_get_result_types($jobid);
if ($outputLevel > 0) {
print STDOUT 'Available result types:', "\n";
}
foreach my $resultType (@resultTypes) {
print STDOUT $resultType->{'identifier'}, "\n";
if (defined($resultType->{'label'})) {
print STDOUT "\t", $resultType->{'label'}, "\n";
}
if (defined($resultType->{'description'})) {
print STDOUT "\t", $resultType->{'description'}, "\n";
}
if (defined($resultType->{'mediaType'})) {
print STDOUT "\t", $resultType->{'mediaType'}, "\n";
}
if (defined($resultType->{'fileSuffix'})) {
print STDOUT "\t", $resultType->{'fileSuffix'}, "\n";
}
}
if ($status eq 'FINISHED' && $outputLevel > 0) {
print STDERR "\n", 'To get results:', "\n",
" perl $scriptName --polljob --jobid " . $params{'jobid'} . "\n",
" perl $scriptName --polljob --outformat <type> --jobid "
. $params{'jobid'} . "\n";
}
}
print_debug_message('result_types', 'End', 1);
}
=head2 submit_job()
Submit a job to the service.
&submit_job($seq);
=cut
sub submit_job {
print_debug_message('submit_job', 'Begin', 1);
# Set input sequence
$params{'sequence'} = shift;
my $seq_id = shift;
# Load parameters
&load_params();
# Submit the job
my $jobid = &rest_run($params{'email'}, $params{'title'}, \%params);
# Asynchronous submission.
if (defined($params{'asyncjob'})) {
print STDOUT $jobid, "\n";
if ($outputLevel > 0) {
print STDERR
"To check status: perl $scriptName --status --jobid $jobid\n";
}
}
# Simulate synchronous submission serial mode.
else {
if ($outputLevel > 0) {
print STDERR "JobId: $jobid\n";
} else {
print STDERR "$jobid\n";
}
usleep($checkInterval);
# Get results.
&get_results($jobid, $seq_id);
}
print_debug_message('submit_job', 'End', 1);
return $jobid;
}
=head2 multi_submit_job()
Submit multiple jobs assuming input is a collection of fasta formatted sequences.
&multi_submit_job();
=cut
sub multi_submit_job {
print_debug_message('multi_submit_job', 'Begin', 1);
my (@filename_list) = ();
# Query sequence
if (defined($ARGV[0])) { # Bare option
if (-f $ARGV[0] || $ARGV[0] eq '-') { # File
push(@filename_list, $ARGV[0]);
}
else {
warn 'Warning: Input file "' . $ARGV[0] . '" does not exist';
}
}
if ($params{'sequence'}) { # Via --sequence
if (-f $params{'sequence'} || $params{'sequence'} eq '-') { # File
push(@filename_list, $params{'sequence'});
}
else {
warn 'Warning: Input file "'
. $params{'sequence'}
. '" does not exist';
}
}
# Job identifier tracking for parallel execution.
my @jobid_list = ();
my $job_number = 0;
$/ = '>';
foreach my $filename (@filename_list) {
my $INFILE;
if ($filename eq '-') { # STDIN.
open($INFILE, '<-')
or die 'Error: unable to STDIN (' . $! . ')';
}
else { # File.
open($INFILE, '<', $filename)
or die 'Error: unable to open file '
. $filename . ' ('
. $! . ')';
}
while (<$INFILE>) {
my $seq = $_;
$seq =~ s/>$//;
if ($seq =~ m/(\S+)/) {
my $seq_id = $1;
print STDERR "Submitting job for: $seq_id\n"
if ($outputLevel > 0);
$seq = '>' . $seq;
&print_debug_message('multi_submit_job', $seq, 11);
$job_number++;
my $job_id = &submit_job($seq, $seq_id);
my $job_info_str = sprintf('%s %d %d', $job_id, 0, $job_number);
push(@jobid_list, $job_info_str);
}
# Parallel mode, wait for job(s) to finish to free slots.
while ($params{'maxJobs'} > 1
&& scalar(@jobid_list) >= $params{'maxJobs'}) {
&_job_list_poll(\@jobid_list);
print_debug_message('multi_submit_job',
'Remaining jobs: ' . scalar(@jobid_list), 1);
}
}
close $INFILE;
}
# Parallel mode, wait for remaining jobs to finish.
while ($params{'maxJobs'} > 1 && scalar(@jobid_list) > 0) {
&_job_list_poll(\@jobid_list);
print_debug_message('multi_submit_job',
'Remaining jobs: ' . scalar(@jobid_list), 1);
}
print_debug_message('multi_submit_job', 'End', 1);
}
=head2 _job_list_poll()
Poll the status of a list of jobs and fetch results for finished jobs.
while(scalar(@jobid_list) > 0) {
&_job_list_poll(\@jobid_list);
}
=cut
sub _job_list_poll {
print_debug_message('_job_list_poll', 'Begin', 1);
my $jobid_list = shift;
print_debug_message('_job_list_poll', 'Num jobs: ' . scalar(@$jobid_list),
11);
# Loop though job Id list polling job status.
for (my $jobNum = (scalar(@$jobid_list) - 1); $jobNum > -1; $jobNum--) {
my ($jobid, $seq_id, $error_count, $job_number) =
split(/\s+/, $jobid_list->[$jobNum]);
print_debug_message('_job_list_poll', 'jobNum: ' . $jobNum, 12);
print_debug_message('_job_list_poll',
'Job info: ' . $jobid_list->[$jobNum], 12);
# Get job status.
my $job_status = &rest_get_status($jobid);
print_debug_message('_job_list_poll', 'Status: ' . $job_status, 12);
# Fetch results and remove finished/failed jobs from list.
if (
!(
$job_status eq 'RUNNING'
|| $job_status eq 'QUEUED'
|| ($job_status eq 'ERROR'
&& $error_count < $maxErrorStatusCount)
)
) {
if ($job_status eq 'ERROR' || $job_status eq 'FAILED') {
print STDERR
"Warning: job $jobid failed for sequence $job_number: $seq_id\n";
}
# Duplicated getting results.
#&get_results($jobid, $seq_id);
splice(@$jobid_list, $jobNum, 1);
}
else {
# Update error count, increment for new error or clear old errors.
if ($job_status eq 'ERROR') {
$error_count++;
}
elsif ($error_count > 0) {
$error_count--;
}
# Update job tracking info.
my $job_info_str = sprintf('%s %s %d %d',
$jobid, $seq_id, $error_count, $job_number);
$jobid_list->[$jobNum] = $job_info_str;
}
}
print_debug_message('_job_list_poll', 'Num jobs: ' . scalar(@$jobid_list),
11);
print_debug_message('_job_list_poll', 'End', 1);
}
=head2 list_file_submit_job()
Submit multiple jobs using a file containing a list of entry identifiers as
input.
&list_file_submit_job($list_filename)
=cut
sub list_file_submit_job {
print_debug_message('list_file_submit_job', 'Begin', 1);
my $filename = shift;
# Open the file of identifiers.
my $LISTFILE;
if ($filename eq '-') { # STDIN.
open($LISTFILE, '<-')
or die 'Error: unable to STDIN (' . $! . ')';
}
else { # File.
open($LISTFILE, '<', $filename)
or die 'Error: unable to open file ' . $filename . ' (' . $! . ')';
}
# Job identifier tracking for parallel execution.
my @jobid_list = ();
my $job_number = 0;
# Iterate over identifiers, submitting each job
while (<$LISTFILE>) {
my $line = $_;
chomp($line);
if ($line ne '') {
&print_debug_message('list_file_submit_job', 'line: ' . $line, 2);
if ($line =~ m/\w:\w/) {
# Check this is an identifier
my $seq_id = $line;
print STDERR "Submitting job for: $seq_id\n"
if ($outputLevel > 0);
$job_number++;
my $job_id = &submit_job($seq_id, $seq_id);
my $job_info_str =
sprintf('%s %s %d %d', $job_id, $seq_id, 0, $job_number);
push(@jobid_list, $job_info_str);
}
else {
print STDERR
"Warning: line \"$line\" is not recognised as an identifier\n";
}
# Parallel mode, wait for job(s) to finish to free slots.
while ($params{'maxJobs'} > 1
&& scalar(@jobid_list) >= $params{'maxJobs'}) {
&_job_list_poll(\@jobid_list);
print_debug_message('list_file_submit_job',
'Remaining jobs: ' . scalar(@jobid_list), 1);
}
}
}
close $LISTFILE;
# Parallel mode, wait for remaining jobs to finish.
while ($params{'maxJobs'} > 1 && scalar(@jobid_list) > 0) {
&_job_list_poll(\@jobid_list);
print_debug_message('list_file_submit_job',
'Remaining jobs: ' . scalar(@jobid_list), 1);
}
print_debug_message('list_file_submit_job', 'End', 1);
}
=head2 load_data()
Load sequence data from file or option specified on the command-line.
&load_data();
=cut
sub load_data {
print_debug_message('load_data', 'Begin', 1);
my $retSeq;
# Query sequence
if (defined($ARGV[0])) { # Bare option
if (-f $ARGV[0] || $ARGV[0] eq '-') { # File
$retSeq = &read_file($ARGV[0]);
}
else { # DB:ID or sequence
$retSeq = $ARGV[0];
}
}
if ($params{'sequence'}) { # Via --sequence
if (-f $params{'sequence'} || $params{'sequence'} eq '-') { # File
$retSeq = &read_file($params{'sequence'});
}
else { # DB:ID or sequence
$retSeq = $params{'sequence'};
}
}
print_debug_message('load_data', 'End', 1);
return $retSeq;
}
=head2 load_params()
Load job parameters from command-line options.
&load_params();
=cut
sub load_params {
print_debug_message('load_params', 'Begin', 1);
# Pass default values and fix bools (without default value)
if (!$params{'goterms'}) {
$params{'goterms'} = 'true'
}
if (!$params{'pathways'}) {
$params{'pathways'} = 'true'
}
print_debug_message('load_params', 'End', 1);
}
=head2 client_poll()
Client-side job polling.
&client_poll($job_id);
=cut
sub client_poll {
print_debug_message('client_poll', 'Begin', 1);
my $jobid = shift;
my $status = 'QUEUED';
# Check status and wait if not finished. Terminate if three attempts get "ERROR".
my $errorCount = 0;