crosscontaminate.sh

#!/bin/bash

usage(){
echo "
Written by Brian Bushnell
Last modified February 17, 2015

Description:  Generates synthetic cross-contaminated files from clean files.
Intended for use with synthetic reads generated by SynthMDA or RandomReads.

Usage:        crosscontaminate.sh in=<file,file,...> out=<file,file,...>

Input parameters:
in=<file,file,...>  Clean input reads.
innamefile=<file>   A file containing the names of input files, 
                    one name per line.
interleaved=auto    (int) t/f overrides interleaved autodetection.
qin=auto            Input quality offset: 33 (Sanger), 64, or auto.
reads=-1            If positive, quit after processing X reads or pairs.

Processing Parameters:
minsinks=1          Min contamination destinations from one source.
maxsinks=8          Max contamination destinations from one source.
minprob=0.000005    Min allowed contamination rate (geometric distribution).
maxprob=0.025       Max allowed contamination rate.

Output parameters:
out=<file,file,...> Contaminated output reads.
outnamefile=<file>  A file containing the names of output files, 
                    one name per line.
overwrite=t         (ow) Grant permission to overwrite files.
#showspeed=t        (ss) 'f' suppresses display of processing speed.
ziplevel=2          (zl) Compression level; 1 (min) through 9 (max).
threads=auto        (t) Set number of threads to use; default is number of 
                    logical processors.
qout=auto           Output quality offset: 33 (Sanger), 64, or auto.
shuffle=f           Shuffle contents of output files.
shufflethreads=3    Use this many threads for shuffling (uses more memory).

Java Parameters:
-Xmx                This will set Java's memory usage, overriding autodetection.
                    -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will specify 200 megs.
                    The max is typically 85% of physical memory.
-eoom               This flag will cause the process to exit if an
                    out-of-memory exception occurs.  Requires Java 8u92+.
-da                 Disable assertions.

Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems.
For documentation and the latest version, visit: https://bbmap.org
"
}

if [ -z "$1" ] || [ "$1" = "-h" ] || [ "$1" = "--help" ]; then
	usage
	exit
fi

resolveSymlinks(){
	SCRIPT="$(cd "$(dirname "$0")" && pwd)/$(basename "$0")"
	while [ -h "$SCRIPT" ]; do
		DIR="$(dirname "$SCRIPT")"
		SCRIPT="$(readlink "$SCRIPT")"
		[ "${SCRIPT#/}" = "$SCRIPT" ] && SCRIPT="$DIR/$SCRIPT"
	done
	DIR="$(cd "$(dirname "$SCRIPT")" && pwd)"
	if [ -f "$DIR/bbtools.jar" ]; then
		CP="$DIR/bbtools.jar"
	else
		CP="$DIR/current/"
	fi
}

setEnv(){
	. "$DIR/javasetup.sh"
	. "$DIR/memdetect.sh"

	parseJavaArgs "--xmx=4g" "--xms=256m" "--percent=42" "--mode=auto" "$@"
	setEnvironment
}

launch() {
	CMD="java $EA $EOOM $SIMD $XMX $XMS -cp $CP synth.CrossContaminate $@"
	echo "$CMD" >&2
	eval $CMD
}

resolveSymlinks
setEnv "$@"
launch "$@"