A particularly large Fastq file could create problems to the server and make us incur in unanticipated costs. There should be a mechanism (ideally both on the client and the server) preventing the processing of extremely large files.
Similarly, an unanticipated error might cause the API to extend the computation time which, again, might incur in unanticipated costs. There should be a mechanism to kill the API if a threshold is exceeded.