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Samtool "% Mapped" is unneccesary #256

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JohnHadish opened this issue Jan 29, 2022 · 0 comments
Open

Samtool "% Mapped" is unneccesary #256

JohnHadish opened this issue Jan 29, 2022 · 0 comments

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@JohnHadish
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The module samtools_index creates a file named ${sample_id}.bam.log. This file is incoporated into the MultiQC report as a column titled "% Mapped":

image

Notice in the above image that there is also a column titled "% Aligned". This column is generated by Hisat2/Star.
These columns present the exact same information, except that they are called separate things and generated by 2 different tools. This is fine with Hisat2, but with Star, the Column created by Samtool's is incorrect:

image

STAR does not keep reads in the SAM file which are not aligned, whereas Hisat2 does. This makes it have a confusing column which says 100% allignment.

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