Cancelling 3 Remaining Tasks After DIA-NN Process

[ambgrav]

Hello!

I've been getting this error after DIA-NN completes, in which 3 tasks do not finish. The peptide and protein reports in the DIA-NN output folder are also all empty - they only have the column header names but no protein groups are present. I am not sure if this is an issue with the search, or if there was not enough peptide in my samples to quantify with DIA-NN. I've copied the error below, and my log file is also attached.

Process 'DIA-NN' finished, exit code: 0
DIA-NN [Work dir: C:\Users\malyl\Desktop\gayani_2024\12-26-2024\LBC482\out10]
C:\Users\malyl\Desktop\Plugins\fragpipe\tools\diann\1.8.2_beta_8\win\dia-nn-plotter.exe diann-output\report.stats.tsv diann-output\report.tsv diann-output\report.pdf
[39560] Failed to execute script dia-nn-plotter
Traceback (most recent call last):
File "dia-nn-plotter.py", line 347, in
File "dia-nn-plotter.py", line 179, in report
File "site-packages\pandas\core\indexes\base.py", line 1520, in droplevel
ValueError: Cannot remove 1 levels from an index with 1 levels: at least one level must be left.
Generating report. Stats, main report, output file: diann-output\report.stats.tsv, diann-output\report.tsv, diann-output\report.pdf
Process 'DIA-NN' finished, exit code: -1
Process returned non-zero exit code, stopping

log_2025-01-01_17-59-08.txt

Thank you!
Amber

[fcyu]

Hi Amber,

There are no IDs from the DIA quant:

[0:38] Number of protein isoforms identified at 1% FDR: 0 (precursor-level), 0 (protein-level) (inference performed using proteotypic peptides only)

Please double check the data and parameters.

Best,

Fengchao