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Since Chrome 105, they launched a feature called Chrome Root Store and Certificate Verifier, which is used to verify certificates independently instead of verifying by the operating system.
This feature will not trust all self-signed certificates, even if they have been added to the Trusted Root Certification Authorities store.
And also according to this article, adding the --enable-features=ChromeRootStoreUsed / --disable-features=ChromeRootStoreUsed flag when starting Chrome will enable (default) / disable this feature.